Chromatin-associated RNA sequencing (ChAR-seq) maps genome-wide RNA-to-DNA contacts.

Autor:	Bell JC; Department of Biochemistry, Stanford University, Stanford, United States., Jukam D; Department of Biology, Stanford University, Stanford, United States., Teran NA; Department of Biochemistry, Stanford University, Stanford, United States.; Department of Genetics, Stanford University, Stanford, United States., Risca VI; Department of Genetics, Stanford University, Stanford, United States., Smith OK; Department of Biochemistry, Stanford University, Stanford, United States.; Department of Chemical and Systems Biology, Stanford University, Stanford, United States., Johnson WL; Department of Biochemistry, Stanford University, Stanford, United States., Skotheim JM; Department of Biology, Stanford University, Stanford, United States., Greenleaf WJ; Department of Genetics, Stanford University, Stanford, United States.; Department of Applied Physics, Stanford University, Stanford, United States., Straight AF; Department of Biochemistry, Stanford University, Stanford, United States.; Department of Chemical and Systems Biology, Stanford University, Stanford, United States.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2018 Apr 12; Vol. 7. Date of Electronic Publication: 2018 Apr 12.
DOI:	10.7554/eLife.27024
Abstrakt:	RNA is a critical component of chromatin in eukaryotes, both as a product of transcription, and as an essential constituent of ribonucleoprotein complexes that regulate both local and global chromatin states. Here, we present a proximity ligation and sequencing method called Ch romatin- A ssociated R NA seq uencing (ChAR-seq) that maps all RNA-to-DNA contacts across the genome. Using Drosophila cells, we show that ChAR-seq provides unbiased, de novo identification of targets of chromatin-bound RNAs including nascent transcripts, chromosome-specific dosage compensation ncRNAs, and genome-wide trans-associated RNAs involved in co-transcriptional RNA processing. Competing Interests: JB, DJ, NT, VR, OS, WJ, JS, WG, AS No competing interests declared (© 2018, Bell et al.)
Databáze:	MEDLINE
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