Adipose-derived stem cells improve full-thickness skin grafts in a rat model.

Autor: Vidor SB; Graduate Program in Veterinary Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil; Laboratory of Embryology and Cell Differentiation, Experimental Research Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil. Electronic address: silvana.b.vidor@gmail.com., Terraciano PB; Laboratory of Embryology and Cell Differentiation, Experimental Research Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil., Valente FS; Graduate Program in Veterinary Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil., Rolim VM; Graduate Program in Veterinary Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil., Kuhl CP; Laboratory of Embryology and Cell Differentiation, Experimental Research Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil., Ayres LS; Laboratory of Embryology and Cell Differentiation, Experimental Research Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil., Garcez TNA; Graduate Program in Veterinary Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil., Lemos NE; Graduate Program in Medical Sciences: Endocrinology, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil., Kipper CE; Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil., Pizzato SB; Laboratory of Embryology and Cell Differentiation, Experimental Research Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil., Driemeier D; Graduate Program in Veterinary Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil., Cirne-Lima EO; Laboratory of Embryology and Cell Differentiation, Experimental Research Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil., Contesini EA; Graduate Program in Veterinary Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Jazyk: angličtina
Zdroj: Research in veterinary science [Res Vet Sci] 2018 Jun; Vol. 118, pp. 336-344. Date of Electronic Publication: 2018 Mar 28.
DOI: 10.1016/j.rvsc.2018.03.014
Abstrakt: To investigate the effects of heterologous adipose-derived stem cells (ADSCs) on autologous full-thickness skin grafts, we designed a first-intention healing model using Wistar rats. We harvested and sutured two full-thickness skin grafts in the dorsal recipient beds of 15 rats, randomized into three groups. In the treatment group, 1 × 10 6 ADSCs resuspended in saline solution (200 μL) were administered subcutaneously to the skin graft. The control group received only saline solution subcutaneously, whereas the negative control group did not receive any treatment. Compressive dressings were maintained until postoperative day 5. The grafts were assessed by two observers, who checked for the presence of epidermolysis on day 14. Planimetry showed the relative areas of normal skin, redness, ulceration, and contraction. Graft samples were obtained on day 14 and stained with hematoxylin and eosin and Masson's trichrome. Epidermal analysis evaluated thickening, keratosis, acanthosis, hydropic degeneration, and inflammatory infiltrate. Dermal evaluation investigated the absence of hair follicles, granulation tissue formation, presence of inflammatory infiltrate, and collagen deposition. Immunohistochemistry was performed for dermal anti-VEGF and epidermal anti-Ki-67 staining. The ADSC group presented better macroscopic aspects, lower incidence of epidermolysis, and less loss of hair follicles. In addition, the ADSC group presented the lowest frequency of histopathological changes in the dermis and epidermis, as well as the largest subcutaneous and granulation tissue VEGF averages and the weakest Ki-67 staining of the epidermal basal layer. Subcutaneous administration of ADSCs may improve the integration of skin grafts, reducing the deleterious effects of ischemia and reperfusion injury.
(Copyright © 2018 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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