High frequency of M. leprae DNA detection in asymptomatic household contacts.

Autor: Gama RS; Universidade Vale do Rio Doce/UNIVALE-Núcleo de Pesquisa em Imunologia, Rua Israel Pinheiro2000, B. Universitário, Governador Valadares, MG, Brazil.; Universidade Federal de Juiz de Fora. Programa de Pós Graduação em Ciências Biológicas (Imunologia e DIP/Genética e Biotecnologia)-Rua José Lourenço Kelmer, S/n-Martelos, Juiz de Fora-MG, Brazil., Gomides TAR; Universidade Vale do Rio Doce/UNIVALE-Núcleo de Pesquisa em Imunologia, Rua Israel Pinheiro2000, B. Universitário, Governador Valadares, MG, Brazil., Gama CFM; Universidade Vale do Rio Doce/UNIVALE-Núcleo de Pesquisa em Imunologia, Rua Israel Pinheiro2000, B. Universitário, Governador Valadares, MG, Brazil., Moreira SJM; FIOCRUZ-Fundação Oswaldo Cruz, Laboratório de Hanseníase, Av. Brasil, Rio de Janeiro, RJ, Brazil., de Neves Manta FS; FIOCRUZ-Fundação Oswaldo Cruz, Laboratório de Hanseníase, Av. Brasil, Rio de Janeiro, RJ, Brazil., de Oliveira LBP; Universidade Vale do Rio Doce/UNIVALE-Núcleo de Pesquisa em Imunologia, Rua Israel Pinheiro2000, B. Universitário, Governador Valadares, MG, Brazil.; Universidade Federal de Juiz de Fora-Campus Governador Valadares-UFJF/GV, Rua Israel Pinheiro, 2000, B. Universitário, Governador Valadares, MG, Brazil., Marçal PHF; Universidade Vale do Rio Doce/UNIVALE-Núcleo de Pesquisa em Imunologia, Rua Israel Pinheiro2000, B. Universitário, Governador Valadares, MG, Brazil.; Universidade Federal de Juiz de Fora. Programa de Pós Graduação em Ciências Biológicas (Imunologia e DIP/Genética e Biotecnologia)-Rua José Lourenço Kelmer, S/n-Martelos, Juiz de Fora-MG, Brazil., Sarno EN; FIOCRUZ-Fundação Oswaldo Cruz, Laboratório de Hanseníase, Av. Brasil, Rio de Janeiro, RJ, Brazil., Moraes MO; FIOCRUZ-Fundação Oswaldo Cruz, Laboratório de Hanseníase, Av. Brasil, Rio de Janeiro, RJ, Brazil., Garcia RMG; Universidade Federal de Juiz de Fora. Programa de Pós Graduação em Ciências Biológicas (Imunologia e DIP/Genética e Biotecnologia)-Rua José Lourenço Kelmer, S/n-Martelos, Juiz de Fora-MG, Brazil., de Oliveira Fraga LA; Universidade Federal de Juiz de Fora-Campus Governador Valadares-UFJF/GV, Rua Israel Pinheiro, 2000, B. Universitário, Governador Valadares, MG, Brazil. luciaalvesfraga@yahoo.com.br.; Universidade Federal de Juiz de Fora. Programa de Pós Graduação em Ciências Biológicas (Imunologia e DIP/Genética e Biotecnologia)-Rua José Lourenço Kelmer, S/n-Martelos, Juiz de Fora-MG, Brazil. luciaalvesfraga@yahoo.com.br.
Jazyk: angličtina
Zdroj: BMC infectious diseases [BMC Infect Dis] 2018 Apr 02; Vol. 18 (1), pp. 153. Date of Electronic Publication: 2018 Apr 02.
DOI: 10.1186/s12879-018-3056-2
Abstrakt: Background: Characterization of the Mycobacterium leprae genome has made possible the development of Polymerase Chain Reaction (PCR) systems that can amplify different genomic regions. Increased reliability and technical efficiency of quantitative PCR (qPCR) makes it a promising tool for early diagnosis of leprosy. Index cases that are multibacillary spread the bacillus silently, even before they are clinically diagnosed. Early detection and treatment could prevent transmission in endemic areas.
Methods: In this study, the qPCR technique is used to detect DNA of M. leprae in samples of slit skin smears (SSS) of the ear lobe and blood of leprosy patients and their asymptomatic household contacts residing in Governador Valadares, MG, Brazil, a hyperendemic area for leprosy. A total of 164 subjects participated in the study: 43 index cases, 113 household contacts, and, as negative controls, 8 individuals who reported no contact with patients nor history of leprosy in the family. The qPCR was performed to amplify 16S rRNA fragments and was specifically designed for M. leprae.
Results: Of asymptomatic household contacts, 23.89% showed bacillary DNA by qPCR in samples of SSS and blood. Also, 48.84% of patients diagnosed with leprosy were positive for qPCR while the bacillary load was positive in only 30.23% of patients. It is important to note that most patients were already receiving treatment when the collection of biological material for qPCR was performed. The level of bacillary DNA from household contacts was similar to the DNA levels detected in the group of paucibacillary patients.
Conclusion: Considering that household contacts comprise a recognizable group of individuals with a high risk of disease, as they live in close proximity to a source of infection, qPCR can be used to estimate the risk of progress towards leprosy among household contacts and as a routine screening method for a chemoprophylactic protocol.
Databáze: MEDLINE
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