Characterizing the behavior, uptake, and toxicity of NM300K silver nanoparticles in Caenorhabditis elegans.

Autor: Kleiven M; Center for Environmental Radioactivity (CERAD CoE), Faculty of Environmental Sciences and Natural Resource Management, Norwegian University of Life Sciences, Ås, Norway., Rossbach LM; Center for Environmental Radioactivity (CERAD CoE), Faculty of Environmental Sciences and Natural Resource Management, Norwegian University of Life Sciences, Ås, Norway., Gallego-Urrea JA; Center for Environmental Radioactivity (CERAD CoE), Faculty of Environmental Sciences and Natural Resource Management, Norwegian University of Life Sciences, Ås, Norway.; Department of Marine Sciences, University of Gothenburg, Kristineberg, Fiskebäckskil, Sweden., Brede DA; Center for Environmental Radioactivity (CERAD CoE), Faculty of Environmental Sciences and Natural Resource Management, Norwegian University of Life Sciences, Ås, Norway., Oughton DH; Center for Environmental Radioactivity (CERAD CoE), Faculty of Environmental Sciences and Natural Resource Management, Norwegian University of Life Sciences, Ås, Norway., Coutris C; Division of Environment and Natural Resources, Norwegian Institute of Bioeconomy Research, Høgskoleveien, Ås, Norway.
Jazyk: angličtina
Zdroj: Environmental toxicology and chemistry [Environ Toxicol Chem] 2018 Jul; Vol. 37 (7), pp. 1799-1810. Date of Electronic Publication: 2018 May 11.
DOI: 10.1002/etc.4144
Abstrakt: Using Caenorhabditis elegans as a model organism, we addressed the potential linkage among toxicity of NM300K Ag nanoparticles (AgNPs), their particle size distribution, and the presence of dissolved Ag in the test media. Of the 3 endpoints assessed (growth, fertility, and reproduction), reproduction was the most sensitive, with the 50% effect concentration (EC50) ranging from 0.26 to 0.84 mg Ag L -1 and 0.08 to 0.11 mg Ag L -1 for NM300K and AgNO 3 , respectively. Silver uptake by C. elegans was similar for both forms of Ag, whereas bioaccumulation was higher in AgNO 3 exposure. The observed differences in toxicity between NM300K and AgNO 3 did not correlate with bioaccumulated Ag, which suggests that toxicity is a function of the type of exposing agent (AgNPs vs AgNO 3 ) and its mode of action. Before addition of the food source (Escherichia coli), size fractionation revealed that dissolved Ag comprised 13 to 90% and 4 to 8% of total Ag in the AgNO 3 and NM300K treatments, respectively. No dissolved Ag was detectable in the actual test media due to immediate Ag adsorption to bacteria. The results of the present study indicate that information on behavior and characterization of exposure conditions is essential for nanotoxicity studies. Environ Toxicol Chem 2018;37:1799-1810. © 2018 SETAC.
(© 2018 SETAC.)
Databáze: MEDLINE
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