Application of nanomaterials for the electrical and optical detection of the hepatitis B virus.

Autor: Oliveira DA; Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, Brazil., Silva JV; Institute of Chemistry, Federal University of Uberlândia, Uberlândia, Brazil., Flauzino JMR; Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, Brazil., Castro ACH; Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, Brazil., Moço ACR; Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, Brazil., Soares MMCN; Adolfo Lutz Institute, Regional Laboratory in São José do Rio Preto, Brazil., Madurro JM; Institute of Chemistry, Federal University of Uberlândia, Uberlândia, Brazil., Brito-Madurro AG; Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, Brazil. Electronic address: agbrito@ufu.br.
Jazyk: angličtina
Zdroj: Analytical biochemistry [Anal Biochem] 2018 May 15; Vol. 549, pp. 157-163. Date of Electronic Publication: 2018 Mar 27.
DOI: 10.1016/j.ab.2018.03.023
Abstrakt: This work describes different approaches for the detection of hepatitis B virus (HBV) genomic DNA, using electrochemical and optical techniques. The platforms consisted of a single-stranded DNA probe (HEPB1S), specific to HBV, grafted on a gold electrode modified with reduced graphene oxide or gold nanoparticles. Differential pulse voltammetry analysis indicates that the addition of HBV genomic DNA caused an increase of about 1.4 times in the current peak value, when compared to the negative control. It was observed a linear dependence with the log HBV-genomic DNA concentration and the electrochemical biosensor detected until 7.65 pg μL -1 of the target. Electrochemical impedance spectroscopy measurements showed an increase of about 2 times in the charge transfer resistance, after the addition of HBV genomic DNA. Assays using colloidal suspension of gold nanoparticles showed a shift of the peak wavelength, linearly proportional to the HBV-genomic DNA concentration, with a detection limit of 0.15 ng μL -1 . The applicability of the gold nanoparticles for clinical samples was tested with success in the blood plasma. All the approaches used in this work were effective in detecting genomic DNA or blood plasma in positive samples for HBV.
(Copyright © 2018 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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