Proteomic analysis of antimicrobial effects of pegylated silver coated carbon nanotubes in Salmonella enterica serovar Typhimurium.

Autor: Park SB; Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State, MS, 39762, USA., Steadman CS; Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State, MS, 39762, USA., Chaudhari AA; Center for Nanobiotechnology Research, Alabama State University, Montgomery, AL, USA., Pillai SR; Center for Nanobiotechnology Research, Alabama State University, Montgomery, AL, USA., Singh SR; Center for Nanobiotechnology Research, Alabama State University, Montgomery, AL, USA., Ryan PL; Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State, MS, 39762, USA.; Department of Pathobiology and Population Medicine, Mississippi State University, Mississippi State, MS, 39762, USA., Willard ST; Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State, MS, 39762, USA.; Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State, MS, 39762, USA., Feugang JM; Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State, MS, 39762, USA. jn181@ads.msstate.edu.
Jazyk: angličtina
Zdroj: Journal of nanobiotechnology [J Nanobiotechnology] 2018 Mar 27; Vol. 16 (1), pp. 31. Date of Electronic Publication: 2018 Mar 27.
DOI: 10.1186/s12951-018-0355-0
Abstrakt: Background: Synthesis of silver nano-compounds with enhanced antimicrobial effects is of great interest for the development of new antibacterial agents. Previous studies have reported the antibacterial properties of pegylated silver-coated carbon nanotubes (pSWCNT-Ag) showing less toxicity in human cell lines. However, the mechanism underlining the pSWCNT-Ag as a bactericidal agent remained unfolded. Here we assessed the pSWCNT-Ag effects against foodborne pathogenic bacteria growth and proteome profile changes.
Results: Measurements of bioluminescent imaging, optical density, and bacteria colony forming units revealed dose-dependent and stronger bactericidal activity of pSWCNT-Ag than their non-pegylated counterparts (SWCNT-Ag). In ovo administration of pSWCNT-Ag or phosphate-buffered saline resulted in comparable chicken embryo development and growth. The proteomic analysis, using two-dimensional electrophoresis combined with matrix assisted laser desorption/ionization time of flight/time of flight mass spectrometry, was performed on control and surviving Salmonella enterica serovar Typhimurium to pSWCNT-Ag. A total of 15 proteins (ten up-regulated and five down-regulated) differentially expressed proteins were identified. Functional analyses showed significant reduction of proteins associated with biofilm formation, nutrient and energy metabolism, quorum sensing and maintenance of cell structure and cell motility in surviving S. Typhimurium. In contrast, proteins associated with oxygen stress, DNA protection, starvation, membrane rebuilding, and alternative nutrient formation were induced as the compensatory reaction.
Conclusions: This study provides further evidence of the antibacterial effects of pSWCNT-Ag nanocomposites and knowledge of their mechanism of action through various protein changes. The findings may lead to the development of more effective and safe antimicrobial agents.
Databáze: MEDLINE
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