Calpastatin phosphorylation regulates radiation-induced calpain activity in glioblastoma.

Autor:	Bassett EA; Department of Radiation Oncology, The Ohio State University Wexner Medical Center, Columbus, OH, USA., Palanichamy K; Department of Radiation Oncology, The Ohio State University Wexner Medical Center, Columbus, OH, USA., Pearson M; Department of Radiation Oncology, The Ohio State University Wexner Medical Center, Columbus, OH, USA., McElroy JP; Center for Biostatistics, Department of Biomedical Informatics, The Ohio State University, Columbus, OH, USA., Haque SJ; Department of Radiation Oncology, The Ohio State University Wexner Medical Center, Columbus, OH, USA., Bell EH; Department of Radiation Oncology, The Ohio State University Wexner Medical Center, Columbus, OH, USA., Chakravarti A; Department of Radiation Oncology, The Ohio State University Wexner Medical Center, Columbus, OH, USA.
Jazyk:	angličtina
Zdroj:	Oncotarget [Oncotarget] 2018 Feb 19; Vol. 9 (18), pp. 14597-14607. Date of Electronic Publication: 2018 Feb 19 (Print Publication: 2018).
DOI:	10.18632/oncotarget.24523
Abstrakt:	Glioblastoma (GBM) is an aggressive, malignant brain tumor that inevitably develops resistance to conventional chemotherapy and radiation treatments. In order to identify signaling pathways involved in the development of radiation resistance, we performed mass spectrometry-based phospho-proteomic profiling of GBM cell lines and normal human astrocytes before and after radiation treatment. We found radiation induced phosphorylation of a number of proteins including calpastatin, specifically in GBM stem cells (GSCs). Herein, we focused on calpastatin, an endogenous inhibitor of calpain proteases. Radiation-induced phosphorylation of calpastatin at Ser-633 within the inhibitory domain was validated with a phospho-specific antibody. In order to test the functional significance of phosphorylated calpastatin, we utilized site-directed mutagenesis to generate phospho-inactive (Ser633Ala) and phospho-mimetic (Ser633Glu) mutant calpastatin. GBM cell lines stably expressing the mutant calpastatin showed that phosphorylation was necessary for radiation-induced calpain activation. We also showed that casein kinase 2, a pro-survival kinase overexpressed in many cancer types, phosphorylated calpastatin at Ser-633. Our results indicate that calpastatin phosphorylation promotes radiation resistance in GBM cells by increasing the activity of calpain proteases, which are known to promote survival and invasion in cancer. Competing Interests: CONFLICTS OF INTEREST The authors have no conflicts of interest to declare.
Databáze:	MEDLINE
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