Cytokines, Antibodies, and Histopathological Profiles during Giardia Infection and Variant-Specific Surface Protein-Based Vaccination.

Autor:	Serradell MC; Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas, Consejo Nacional de Investigaciones Científicas y Técnicas, Cordoba, Argentina., Gargantini PR; Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas, Consejo Nacional de Investigaciones Científicas y Técnicas, Cordoba, Argentina.; Facultad de Ciencias de la Salud, Escuela de Medicina, Universidad Católica de Córdoba, Cordoba, Argentina., Saura A; Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas, Consejo Nacional de Investigaciones Científicas y Técnicas, Cordoba, Argentina.; Facultad de Ciencias de la Salud, Escuela de Medicina, Universidad Católica de Córdoba, Cordoba, Argentina., Oms SR; Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas, Consejo Nacional de Investigaciones Científicas y Técnicas, Cordoba, Argentina., Rupil LL; Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas, Consejo Nacional de Investigaciones Científicas y Técnicas, Cordoba, Argentina.; Facultad de Ciencias de la Salud, Escuela de Medicina, Universidad Católica de Córdoba, Cordoba, Argentina., Berod L; Institute of Infection Immunology, Twincore, Centre for Experimental and Clinical Infection Research, Hannover Medical School and Helmholtz Centre for Infection Research, Hannover, Germany., Sparwasser T; Institute of Infection Immunology, Twincore, Centre for Experimental and Clinical Infection Research, Hannover Medical School and Helmholtz Centre for Infection Research, Hannover, Germany., Luján HD; Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas, Consejo Nacional de Investigaciones Científicas y Técnicas, Cordoba, Argentina hlujan@cidie.ucc.edu.ar.; Facultad de Ciencias de la Salud, Escuela de Medicina, Universidad Católica de Córdoba, Cordoba, Argentina.
Jazyk:	angličtina
Zdroj:	Infection and immunity [Infect Immun] 2018 May 22; Vol. 86 (6). Date of Electronic Publication: 2018 May 22 (Print Publication: 2018).
DOI:	10.1128/IAI.00773-17
Abstrakt:	Giardiasis is one of the most common human intestinal diseases worldwide. Several experimental animal models have been used to evaluate Giardia infections, with gerbils ( Meriones unguiculatus ) being the most valuable model due to their high susceptibility to Giardia infection, abundant shedding of cysts, and pathophysiological alterations and signs of disease similar to those observed in humans. Here, we report cytokine and antibody profiles both during the course of Giardia infection in gerbils and after immunization with a novel oral vaccine comprising a mixture of purified variant-specific surface proteins (VSPs). Transcript levels of representative cytokines of different immune profiles as well as macro- and microtissue alterations were assessed in Peyer's patches, mesenteric lymph nodes, and spleens. During infection, cytokine responses showed a biphasic profile: an early induction of Th1 (gamma interferon [IFN-γ], interleukin-1β [IL-1β], IL-6, and tumor necrosis factor [TNF]), Th17 (IL-17), and Th2 (IL-4) cytokines, together with intestinal alterations typical of inflammation, followed by a shift toward a predominant Th2 (IL-5) response, likely associated with a counterregulatory mechanism. Conversely, immunization with an oral vaccine comprising the entire repertoire of VSPs specifically showed high levels of IL-17, IL-6, IL-4, and IL-5, without obvious signs of inflammation. Both immunized and infected animals developed local (intestinal secretory IgA [S-IgA]) and systemic (serum IgG) humoral immune responses against VSPs; however, only infected animals showed evident signs of giardiasis. This is the first comprehensive report of cytokine expression and anti- Giardia antibody production during infection and VSP vaccination in gerbils, a reliable model of the human disease. (Copyright © 2018 American Society for Microbiology.)
Databáze:	MEDLINE
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