Genetic diversity and population structure of Theileria parva in South Sudan.

Autor: Salih DA; Biosciences Eastern and Central Africa-International Livestock Research Institute Hub (BecA-ILRI Hub), P.O. Box 30709-00100, Nairobi, Kenya; Central Veterinary Research Laboratory, P.O. Box 8067, Khartoum, Sudan. Electronic address: diaeldin2000@hotmail.com., Mwacharo JM; School of Life Sciences, Centre for Genetics and Genomics, University of Nottingham, Nottingham, NG7 2RD, UK., Pelle R; Biosciences Eastern and Central Africa-International Livestock Research Institute Hub (BecA-ILRI Hub), P.O. Box 30709-00100, Nairobi, Kenya., Njahira MN; Biosciences Eastern and Central Africa-International Livestock Research Institute Hub (BecA-ILRI Hub), P.O. Box 30709-00100, Nairobi, Kenya., Odongo DO; International Livestock Research Institute (ILRI), P.O. Box 30709-00100, Nairobi, Kenya, Kenya; School of Biological Sciences, The University of Nairobi, P.O. Box 30197, Nairobi, 00100, Kenya., Mbole-Kariuki MN; International Livestock Research Institute (ILRI), P.O. Box 30709-00100, Nairobi, Kenya, Kenya., Marcellino WL; Ministry of Animal Resources and Fisheries, P.O. Box 126, Juba, South Sudan., Malak AK; Ministry of Animal Resources and Fisheries, P.O. Box 126, Juba, South Sudan., Kiara H; International Livestock Research Institute (ILRI), P.O. Box 30709-00100, Nairobi, Kenya, Kenya., El Hussein ARM; Central Veterinary Research Laboratory, P.O. Box 8067, Khartoum, Sudan., Bishop RP; International Livestock Research Institute (ILRI), P.O. Box 30709-00100, Nairobi, Kenya, Kenya., Skilton RA; Biosciences Eastern and Central Africa-International Livestock Research Institute Hub (BecA-ILRI Hub), P.O. Box 30709-00100, Nairobi, Kenya.
Jazyk: angličtina
Zdroj: Ticks and tick-borne diseases [Ticks Tick Borne Dis] 2018 May; Vol. 9 (4), pp. 806-813. Date of Electronic Publication: 2018 Mar 03.
DOI: 10.1016/j.ttbdis.2018.03.002
Abstrakt: Theileria parva is a parasitic protozoan that causes East Coast fever (ECF), an economically important disease of cattle in eastern, central and southern Africa. In South Sudan, ECF is considered a major constraint for livestock development in regions where the disease is endemic. To obtain insights into the dynamics of T. parva in South Sudan, population genetic analysis was performed. Out of the 751 samples included in this study, 178 blood samples were positive for T. parva by species-specific PCR, were collected from cattle from four regions in South Sudan (Bor = 62; Juba = 45; Kajo keji = 41 and Yei = 30) were genotyped using 14 microsatellite markers spanning the four chromosomes. The T. parva Muguga strain was included in the study as a reference. Linkage disequilibrium was evident when populations from the four regions were treated as a single entity, but, when populations were analyzed separately, linkage disequilibrium was observed in Bor, Juba and Kajo keji. Juba region had a higher multiplicity of infection than the other three regions. Principal components analysis revealed a degree of sub-structure between isolates from each region, suggesting that populations are partially distinct, with genetic exchange and gene flow being limited between parasites in the four geographically separated populations studied. Panmixia was observed within individual populations. Overall T. parva population genetic analyses of four populations in South Sudan exhibited a low level of genetic exchange between the populations, but a high level of genetic diversity within each population.
(Copyright © 2018 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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