Swift fabrication of Ag nanostructures using a colloidal solution of Holostemma ada-kodien (Apocynaceae) - Antibiofilm potential, insecticidal activity against mosquitoes and non-target impact on water bugs.

Autor: Alyahya SA; National Center for Biotechnology, King Abdulaziz City for Science and Technology, Riyadh 11442, Saudi Arabia., Govindarajan M; Unit of Vector Control, Phytochemistry and Nanotechnology, Department of Zoology, Annamalai University, Annamalainagar 608 002, Tamil Nadu, India; Department of Zoology, Government College for Women, Kumbakonam 612 001, Tamil Nadu, India. Electronic address: drgovind1979@gmail.com., Alharbi NS; Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia., Kadaikunnan S; Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia., Khaled JM; Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia., Mothana RA; Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia., Al-Anbr MN; Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia., Vaseeharan B; Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health and Management, Alagappa University, Science Block, 6th floor, Burma Colony, Karaikudi 630 003, Tamil Nadu, India., Ishwarya R; Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health and Management, Alagappa University, Science Block, 6th floor, Burma Colony, Karaikudi 630 003, Tamil Nadu, India., Yazhiniprabha M; Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health and Management, Alagappa University, Science Block, 6th floor, Burma Colony, Karaikudi 630 003, Tamil Nadu, India., Benelli G; Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124 Pisa, Italy; The BioRobotics Institute, Sant'Anna School of Advanced Studies, viale Rinaldo Piaggio 34, 56025 Pontedera, Pisa, Italy. Electronic address: benelli.giovanni@gmail.com.
Jazyk: angličtina
Zdroj: Journal of photochemistry and photobiology. B, Biology [J Photochem Photobiol B] 2018 Apr; Vol. 181, pp. 70-79. Date of Electronic Publication: 2018 Feb 17.
DOI: 10.1016/j.jphotobiol.2018.02.019
Abstrakt: Recent research in entomology and parasitology focused on the efficacy of green fabricated nanomaterials as novel insecticides. In this study, we synthesized poly-dispersed and stable silver nanoparticles (AgNPs) using the leaf extract of Holostemma ada-kodien. The nanostructures were characterized by ultraviolet-visible spectroscopy, Fourier-transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy, energy dispersive X-ray, and X-ray diffraction analysis. The efficacy of H. ada-kodien leaf extract and AgNPs in vector control was evaluated against the mosquitoes Anopheles stephensi, Aedes aegypti, and Culex quinquefasciatus, which act as major vectors of important parasitic and arboviral diseases. AgNPs showed higher toxicity if compared to the H. ada-kodien leaf aqueous extract, LC 50 towards larvae of A. stephensi, A. aegypti, and C. quinquefasciatus were 12.18, 13.30, and 14.70 μg/mL, respectively. When the AgNPs were tested on non-target water bugs, Diplonychus indicus, the LC 50 value was 623.48 μg/mL. Furthermore, 100 μl/mL of AgNPs achieved significant antimicrobial activity against Bacillus pumilus, Enterococcus faecalis, Pseudomonas aeruginosa, Proteus vulgaris, and Candida albicans. Light and confocal laser scanning microscopy highlighted a major impact of the H. ada-kodien-synthesized AgNPs on the external topography and architecture of microbial biofilms, both on Gram-positive and Gram-negative bacteria. Overall, this study sheds light on the insecticidal and antibiofilm potential of H. ada-kodien-synthesized AgNPs, a potential green resource for the rapid synthesis of polydispersed and highly stable AgNPs.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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