| Autor: |
De Magalhaes Filho CD; The Howard Hughes Medical Institute, Molecular and Cell Biology Department, Li Ka Shing Center, University of California Berkeley, Berkeley, CA, 94720, USA.; The Salk Institute for Biological Studies, Gene expression laboratory, The Howard Hughes Medical Institute, 10010 N.Torrey Pines Road, La Jolla, CA, 92037, USA., Henriquez B; The Salk Institute for Biological Studies, Gene expression laboratory, The Howard Hughes Medical Institute, 10010 N.Torrey Pines Road, La Jolla, CA, 92037, USA., Seah NE; Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI, 02912, USA., Evans RM; The Salk Institute for Biological Studies, Gene expression laboratory, The Howard Hughes Medical Institute, 10010 N.Torrey Pines Road, La Jolla, CA, 92037, USA., Lapierre LR; Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI, 02912, USA., Dillin A; The Howard Hughes Medical Institute, Molecular and Cell Biology Department, Li Ka Shing Center, University of California Berkeley, Berkeley, CA, 94720, USA. dillin@berkeley.edu. |
| Abstrakt: |
The transparent nematode Caenorhabditis elegans can sense UV and blue-violet light to alter behavior. Because high-dose UV and blue-violet light are not a common feature outside of the laboratory setting, we asked what role, if any, could low-intensity visible light play in C. elegans physiology and longevity. Here, we show that C. elegans lifespan is inversely correlated to the time worms were exposed to visible light. While circadian control, lite-1 and tax-2 do not contribute to the lifespan reduction, we demonstrate that visible light creates photooxidative stress along with a general unfolded-protein response that decreases the lifespan. Finally, we find that long-lived mutants are more resistant to light stress, as well as wild-type worms supplemented pharmacologically with antioxidants. This study reveals that transparent nematodes are sensitive to visible light radiation and highlights the need to standardize methods for controlling the unrecognized biased effect of light during lifespan studies in laboratory conditions. |
