An Amperometric Biosensor for the Determination of Bacterial Sepsis Biomarker, Secretory Phospholipase Group 2-IIA Using a Tri-Enzyme System.

Autor: Nik Mansor NN; School of Chemical Sciences and Food Technology, Faculty of Science & Technology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor DE, Malaysia. niknurhanan@gmail.com., Leong TT; Department of Emergency Medicine, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras 56000, Kuala Lumpur, Malaysia. sebastianttl@yahoo.co.uk., Safitri E; Department of Chemistry, Faculty of Mathematics and Natural Sciences, Syiah Kuala University (USK), Darussalam-Banda Acheh 23111, Indonesia. e.safitri@yahoo.co.id., Futra D; The Department of Chemistry Education, Faculty of Education, Universitas Riau, Kampus Binawidya KM 12.5, Pekanbaru 28293, Riau, Indonesia. futra.dedi@yahoo.com., Ahmad NS; Department of Emergency Medicine, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras 56000, Kuala Lumpur, Malaysia. saadah182@gmail.com., Nasuruddin DN; Department of Pathology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras 56000, Kuala Lumpur, Malaysia. dal.nuun@gmail.com., Itnin A; Department of Pathology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras 56000, Kuala Lumpur, Malaysia. nazlin@ppukm.ukm.edu.my., Zaini IZ; Department of Emergency Medicine, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras 56000, Kuala Lumpur, Malaysia. ida_zarina74@yahoo.com., Arifin KT; Department of Biochemistry Education, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras 56000, Kuala Lumpur, Malaysia. ktarifin@yahoo.com., Heng LY; School of Chemical Sciences and Food Technology, Faculty of Science & Technology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor DE, Malaysia. leeyookheng@yahoo.co.uk., Hassan NI; School of Chemical Sciences and Food Technology, Faculty of Science & Technology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor DE, Malaysia. drizz@ukm.edu.my.
Jazyk: angličtina
Zdroj: Sensors (Basel, Switzerland) [Sensors (Basel)] 2018 Feb 26; Vol. 18 (3). Date of Electronic Publication: 2018 Feb 26.
DOI: 10.3390/s18030686
Abstrakt: A tri-enzyme system consisting of choline kinase/choline oxidase/horseradish peroxidase was used in the rapid and specific determination of the biomarker for bacterial sepsis infection, secretory phospholipase Group 2-IIA (sPLA2-IIA). These enzymes were individually immobilized onto the acrylic microspheres via succinimide groups for the preparation of an electrochemical biosensor. The reaction of sPLA2-IIA with its substrate initiated a cascading enzymatic reaction in the tri-enzyme system that led to the final production of hydrogen peroxide, which presence was indicated by the redox characteristics of potassium ferricyanide, K₃Fe(CN)₆. An amperometric biosensor based on enzyme conjugated acrylic microspheres and gold nanoparticles composite coated onto a carbon-paste screen printed electrode (SPE) was fabricated and the current measurement was performed at a low potential of 0.20 V. This enzymatic biosensor gave a linear range 0.01-100 ng/mL ( R ² = 0.98304) with a detection limit recorded at 5 × 10 -3 ng/mL towards sPLA2-IIA. Moreover, the biosensor showed good reproducibility (relative standard deviation (RSD) of 3.04% ( n = 5). The biosensor response was reliable up to 25 days of storage at 4 °C. Analysis of human serum samples for sPLA2-IIA indicated that the biosensor has potential for rapid bacterial sepsis diagnosis in hospital emergency department.
Competing Interests: The authors declare no conflict of interest.
Databáze: MEDLINE
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