Spectroscopic analyses and genotoxicity of dioxins in the aquatic environment of Alexandria.

Autor: Abdel-Gawad FK; Environmental Research Division, Centre of Excellence for Advanced Science, National Research Centre, 33 El-Bohouth St., 12622 Dokki, Giza, Egypt., Osman O; Spectroscopy Department, National Research Centre, 33 El-Bohouth St., 12622 Dokki, Giza, Egypt., Bassem SM; Environmental Research Division, Centre of Excellence for Advanced Science, National Research Centre, 33 El-Bohouth St., 12622 Dokki, Giza, Egypt., Nassar HF; Environmental Sciences and Industrial Development Department, Faculty of Postgraduate Studies for Advanced Sciences (PSAS), Beni-Suef University, Beni-Suef, Egypt., Temraz TA; Marine Science Department, Faculty of Science, Suez Canal University, Ismailia, Egypt., Elhaes H; Faculty of Women for Arts, Science and Education, Physics Department, Ain Shams University, 11757 Cairo, Egypt., Ibrahim M; Spectroscopy Department, National Research Centre, 33 El-Bohouth St., 12622 Dokki, Giza, Egypt. Electronic address: methat.ibrahim@gmail.com.
Jazyk: angličtina
Zdroj: Marine pollution bulletin [Mar Pollut Bull] 2018 Feb; Vol. 127, pp. 618-625. Date of Electronic Publication: 2018 Jan 05.
DOI: 10.1016/j.marpolbul.2017.12.056
Abstrakt: Dioxins have global concerns because of the bioaccumulation tendency and persistency in the environment. Water, seabream Pagrus auratus and seabass Dicentrarchus labrax samples were collected from Abu Qir, Alexandria to evaluate the concentration of dioxin. Fourier Transform Infrared Spectrometer (FTIR) and molecular modeling was applied for elucidating the molecular structure of fish samples. Furthermore, HPLC with UV detection was used to determine the concentration of dioxins (2,8-dichloro dibenzo-p-dioxin). RT-PCR assay was conducted to verify the expression of some immune genes in the fish species as a result of water pollution. The average detected concentrations varied from 0.2 to 1.3μg/l. Gene expression revealed that MHC class 1 and C3 were highly upregulated in liver and muscle of seabass and seabream while T2BP was highly regulated in seabass liver and seabream muscle and seabass muscle for transferrin, FTIR and molecular modeling indicate that dioxin finds its way to fish protein.
(Copyright © 2017 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: