Development of a new screening method to determine the main 52 mitochondrial haplogroups through a single minisequencing reaction.

Autor: Palencia-Madrid L; BIOMICs Research Group, Lascaray Research Center, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain., Cardoso S; BIOMICs Research Group, Lascaray Research Center, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain., Castro-Maestre F; BIOMICs Research Group, Lascaray Research Center, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain., Baroja-Careaga I; BIOMICs Research Group, Lascaray Research Center, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain., Rocandio AM; Department of Nutrition and Food Sciences, Faculty of Pharmacy, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain., de Pancorbo MM; BIOMICs Research Group, Lascaray Research Center, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain. Electronic address: marian.mdepancorbo@ehu.eus.
Jazyk: angličtina
Zdroj: Mitochondrion [Mitochondrion] 2019 Mar; Vol. 45, pp. 46-51. Date of Electronic Publication: 2018 Feb 21.
DOI: 10.1016/j.mito.2018.02.004
Abstrakt: This work presents the design, development and optimization of a screening method based on single-base extension sequencing to simultaneously analyze a panel of 52 mitochondrial SNPs. This enables to recognize the main mitochondrial haplogroups and to discriminate even between lineages from the same phylogenetic branch that diverged in different continents. The unavailability of individuals harboring infrequent variants was a limitation to optimize the panel. To overcome this, we have modified DNA by site-directed mutagenesis to create the unavailable allelic variants. This allowed us to verify the reliability of this panel and its usefulness to be applied in biomedicine, forensic and population genetic studies.
(Copyright © 2018 Elsevier B.V. and Mitochondria Research Society. All rights reserved.)
Databáze: MEDLINE
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