Plasma Lipidomic Signature of Rectal Adenocarcinoma Reveals Potential Biomarkers.

Autor: Fernandes Messias MC; Laboratory of Multidisciplinary Research, São Francisco University (USF), Bragança Paulista, São Paulo, Brazil., Mecatti GC; Laboratory of Multidisciplinary Research, São Francisco University (USF), Bragança Paulista, São Paulo, Brazil., Figueiredo Angolini CF; Institute of Chemistry, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil., Eberlin MN; Institute of Chemistry, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil., Credidio L; Department of Surgery, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil., Real Martinez CA; Department of Surgery, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil., Rodrigues Coy CS; Department of Surgery, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil., de Oliveira Carvalho P; Laboratory of Multidisciplinary Research, São Francisco University (USF), Bragança Paulista, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Frontiers in oncology [Front Oncol] 2018 Jan 08; Vol. 7, pp. 325. Date of Electronic Publication: 2018 Jan 08 (Print Publication: 2017).
DOI: 10.3389/fonc.2017.00325
Abstrakt: Background: Rectal adenocarcinoma (RAC) is a common malignant tumor of the digestive tract and survival is highly dependent upon stage of disease at diagnosis. Lipidomic strategy can be used to identify potential biomarkers for establishing early diagnosis or therapeutic programs for RAC.
Objective: To evaluate the lipoperoxidation biomarkers and lipidomic signature in the plasma of patients with RAC ( n  = 23) and healthy controls ( n  = 18).
Methods: Lipoperoxidation was evaluated based on malondialdehyde (MDA) and F 2 -isoprostane levels and the lipidomic profile obtained by gas chromatography and high resolution mass spectrometry (ESI-q-TOF) associated with a multivariate statistical technique.
Results: The most abundant ions identified in the RAC patients were those of protonated phosphatidylcholine and phosphatidylethanolamine. It was found that a lisophosphatidylcholine (LPC) plasmalogen containing palmitoleic acid [LPC (P-16:1)], with highest variable importance projection score, showed a tendency to be lower in the cancer patients. A reduction of n -  3 polyunsaturated fatty acids was observed in the plasma of these patients. MDA levels were higher in patients with advanced cancer (stages III/IV) than in the early stages groups and the healthy group ( p  < 0.05). No differences in F 2 -isoprostane levels were observed among these groups.
Conclusion: This study shows that the reduction in plasma levels of LPC plasmalogens associated with an increase in MDA levels may indicate increased oxidative stress in these patients and identify the metabolite LPC (P-16:1) as a putatively novel lipid signature for RAC.
Databáze: MEDLINE
Externí odkaz: