Can artificial tears prevent Acanthamoeba keratitis? An in vitro approach.

Autor: Magnet A; Facultad de Farmacia, Universidad San Pablo CEU, CEU Universities, Madrid, Spain., Gomes TS; Facultad de Farmacia, Universidad San Pablo CEU, CEU Universities, Madrid, Spain.; CAPES Foundation, Ministry of Education of Brazil, Brasília, DF, 70040-020, Brazil., Pardinas C; Facultad de Farmacia, Universidad San Pablo CEU, CEU Universities, Madrid, Spain., Garcia de Blas N; Facultad de Farmacia, Universidad San Pablo CEU, CEU Universities, Madrid, Spain., Sadaba C; Facultad de Medicina, Universidad San Pablo CEU, CEU Universities, Madrid, Spain., Carrillo E; Instituto de Salud Carlos III, Centro Nacional de Microbiología, WHO Collaborating Centre for Leishmaniasis, Madrid, Spain., Izquierdo F; Facultad de Farmacia, Universidad San Pablo CEU, CEU Universities, Madrid, Spain., Del Castillo JMB; Servicio de Oftalmología, Hospital Clínico San Carlos, Universidad Complutense, Madrid, Spain., Hurtado C; Facultad de Farmacia, Universidad San Pablo CEU, CEU Universities, Madrid, Spain., Del Aguila C; Facultad de Farmacia, Universidad San Pablo CEU, CEU Universities, Madrid, Spain., Fenoy S; Facultad de Farmacia, Universidad San Pablo CEU, CEU Universities, Madrid, Spain. sfenrod@ceu.es.
Jazyk: angličtina
Zdroj: Parasites & vectors [Parasit Vectors] 2018 Jan 22; Vol. 11 (1), pp. 50. Date of Electronic Publication: 2018 Jan 22.
DOI: 10.1186/s13071-018-2639-5
Abstrakt: Background: The use of contact lenses has increased in recent years as has the incidence of Dry Eye Syndrome, partly due to their use. Artificial tears are the most common treatment option. Since these changes can facilitate Acanthamoeba infection, the present study has been designed to evaluate the effect of three artificial tears treatments in the viability of Acanthamoeba genotype T4 trophozoites. Optava Fusion™, Oculotect®, and Artelac® Splash were selected due to their formulation.
Methods: Viability was assessed using two staining methods, Trypan Blue stain and CTC stain at different time intervals (2, 4, 6, 8 and 24 h). Trypan Blue viability was obtained by manual count with light microscopy while the CTC stain was determined using flow cytometry.
Results: Trypan Blue staining results demonstrated a decrease in viability for Optava Fusion™ and Artelac® Splash during the first 4 h of incubation. After, this effect seems to lose strength. In the case of Oculotect®, complete cell death was observed after 2 h. Using flow cytometry analysis, Optava Fusion™ and Oculotect® exhibited the same effect observed with Trypan Blue staining. However, Artelac® Splash revealed decreasing cell respiratory activity after four hours, with no damage to the cell membrane.
Conclusions: The present study uses, for the first time, CTC stain analyzed by flow cytometry to establish Acanthamoeba viability demonstrating its usefulness and complementarity with the traditional stain, Trypan Blue. Artelac® Splash, with no preservatives, and Optava Fusion TM, with Purite®, have not shown any useful amoebicidal activity. On the contrary, promising results presented by Ocultect®, with BAK, open up a new possibility for Acanthamoeba keratitis prophylaxis and treatment although in vivo studies should be carried out.
Databáze: MEDLINE
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