Biosynthesis, characterization and leishmanicidal activity of a biocomposite containing AgNPs-PVP-glucantime.

Autor: Gélvez APC; Postgraduate Program in Biology of Infectious & Parasitic Agents, Federal University of Pará, Belém, Pará, Brazil.; Laboratory of Electron Microscopy, Evandro Chagas Institute, Ministry of Health, Belém, Pará, Brazil., Farias LHS; Department of Biomedicine, ESAMAZ, Belém, Pará, Brazil., Pereira VS; Department of Mechanical Engineering, Federal University of Pará, Belém, Pará, Brazil., da Silva ICM; Faculty of Chemical Engineering, Federal University of Pará, Belém,Pará, Brazil., Costa AC; Physico-Chemical Drug Quality Control Laboratory, Federal University of Pará, Belém, Pará, Brazil., Dias CGBT; Department of Mechanical Engineering, Federal University of Pará, Belém, Pará, Brazil., Costa RMR; Physico-Chemical Drug Quality Control Laboratory, Federal University of Pará, Belém, Pará, Brazil., da Silva SHM; Section of Bacteriology & Mycology, Evandro Chagas Institute, Ministry of Health, Ananindeua, Pará, Brazil., Rodrigues APD; Laboratory of Electron Microscopy, Evandro Chagas Institute, Ministry of Health, Belém, Pará, Brazil.
Jazyk: angličtina
Zdroj: Nanomedicine (London, England) [Nanomedicine (Lond)] 2018 Feb; Vol. 13 (4), pp. 373-390. Date of Electronic Publication: 2018 Jan 17.
DOI: 10.2217/nnm-2017-0285
Abstrakt: Aim: Development of functionalized nanocomposites containing AgNPs-PVP-Glucantime ® to evaluate their leishmanicidal activity as a novel method for improving the pharmacological properties of the drug Glucantime ® against extracellular promastigotes and intracellular amastigotes of Leishmania amazonensis in vitro to treat cutaneous leishmaniasis.
Materials & Methods: The silver nanoparticles and nanocomposites prepared containing silver nanoparticles, polyvinylpyrrolidone and different amounts of Glucantime were characterized using transmission electron microscopy, x-ray diffraction, energy-dispersive x-ray spectroscopy and ζ potential analysis; in addition, the in vitro cytotoxicity was evaluated.
Results: The nanocomposites showed an inhibitory effect on the cellular viability of promastigote forms, with values of 47.06, 51.71 and 65.67% for nanocomposite 1 , nanocomposite 2 and nanocomposite 3 , respectively, as well as a dose-dependent decrease in the infectivity index, with values of 33.33 and 23% for nanocomposite 2 and nanocomposite 3 , respectively.
Conclusion: The proposed nanocomposite reveals leishmanial activity and the absence of cytotoxicity in macrophages. Further investigations will be conducted in vivo.
Databáze: MEDLINE
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