| Autor: |
Srinivasan R; Department of Biotechnology, Science Campus, Alagappa University, Karaikudi, Tamil Nadu, -630 003, India., Vigneshwari L; Department of Biotechnology, Science Campus, Alagappa University, Karaikudi, Tamil Nadu, -630 003, India., Rajavel T; Department of Biotechnology, Science Campus, Alagappa University, Karaikudi, Tamil Nadu, -630 003, India., Durgadevi R; Department of Biotechnology, Science Campus, Alagappa University, Karaikudi, Tamil Nadu, -630 003, India., Kannappan A; Department of Biotechnology, Science Campus, Alagappa University, Karaikudi, Tamil Nadu, -630 003, India., Balamurugan K; Department of Biotechnology, Science Campus, Alagappa University, Karaikudi, Tamil Nadu, -630 003, India., Pandima Devi K; Department of Biotechnology, Science Campus, Alagappa University, Karaikudi, Tamil Nadu, -630 003, India., Veera Ravi A; Department of Biotechnology, Science Campus, Alagappa University, Karaikudi, Tamil Nadu, -630 003, India. aveeraravi@rediffmail.com. |
| Abstrakt: |
Urinary tract infections are the utmost common bacterial infections caused by Proteus mirabilis, Pseudomonas aeruginosa, Escherichia coli, and Serratia marcescens. These uropathogens resist the action of several antibiotics due to their ability to form biofilms. Most of these bacterial pathogens use the quorum sensing (QS) machinery to co-ordinate their cells and regulate several virulence factors and biofilm formation. On the other hand, the anti-quorum sensing (anti-QS) and antibiofilm potential of silver nanoparticles have been well reported against certain bacterial pathogens, but to the best of our knowledge, no report is available against the pathogenicity of uropathogens in particular S. marcescens and P. mirabilis. Therefore, the present study is primarily focused on the anti-QS and antibiofilm potential of Piper betle-based synthesized silver nanoparticles (PbAgNPs) against S. marcescens and P. mirabilis. Initially, the silver nanoparticles were synthesized by the aqueous extract of P. betle and characterized by UV-absorbance spectroscopy, XRD, FT-IR, SEM, TEM, and DLS. The synthesized silver nanoparticles were assessed for their anti-QS activity and the obtained results revealed that the PbAgNPs inhibited the QS-mediated virulence factors such as prodigiosin, protease, biofilm formation, exopolysaccharides and hydrophobicity productions in uropathogens. The gene expression analysis divulged the downregulation of fimA, fimC, flhD, and bsmB genes in S. marcescens and flhB, flhD, and rsbA genes in P. mirabilis, respectively. The in vivo Caenorhabditis elegans assays revealed the non-toxic and anti-adherence efficiency of PbAgNPs. Furthermore, the non-toxic effect of PbAgNPs was also confirmed through peripheral blood mononuclear cells and normal lung epithelial cells. Therefore, the contemporary study demonstrates the use of PbAgNPs as a possible alternative toward conventional antibiotics in controlling QS and biofilm-related uropathogen infections. |
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