Comparative genomics identifies male accessory gland proteins in five Glossina species.

Autor:	Abry MF; Center for Biotechnology and Bioinformatics, University of Nairobi, P.O. Box 30197, Nairobi, 00100, Kenya.; International Centre for Insect Physiology and Ecology, P.O. Box 30772, Nairobi, 00100, Kenya., Kimenyi KM; Center for Biotechnology and Bioinformatics, University of Nairobi, P.O. Box 30197, Nairobi, 00100, Kenya.; International Centre for Insect Physiology and Ecology, P.O. Box 30772, Nairobi, 00100, Kenya., Masiga D; International Centre for Insect Physiology and Ecology, P.O. Box 30772, Nairobi, 00100, Kenya., Kulohoma BW; Center for Biotechnology and Bioinformatics, University of Nairobi, P.O. Box 30197, Nairobi, 00100, Kenya.; International Centre for Insect Physiology and Ecology, P.O. Box 30772, Nairobi, 00100, Kenya.
Jazyk:	angličtina
Zdroj:	Wellcome open research [Wellcome Open Res] 2017 Nov 22; Vol. 2, pp. 73. Date of Electronic Publication: 2017 Nov 22 (Print Publication: 2017).
DOI:	10.12688/wellcomeopenres.12445.2
Abstrakt:	Accessory gland proteins (ACPs) are important reproductive proteins produced by the male accessory glands (MAGs) of most insect species. These proteins are essential for male insect fertility, and are transferred alongside semen to females during copulation. ACPs are poorly characterized in Glossina species (tsetse fly), the principal vector of the parasite that causes life-threatening Human African Trypanosomiasis and Animal trypanosomiasis in endemic regions in Africa. The tsetse fly has a peculiar reproductive cycle because of the absence of oviposition. Females mate once and store sperm in a spermathecal, and produce a single fully developed larva at a time that pupates within minutes of exiting their uterus. This slow reproductive cycle, compared to other insects, significantly restricts reproduction to only 3 to 6 larvae per female lifespan. This unique reproductive cycle is an attractive vector control strategy entry point. We exploit comparative genomics approaches to explore the diversity of ACPs in the recently available whole genome sequence data from five tsetse fly species ( Glossina morsitans, G. austeni, G. brevipalpis, G. pallidipes and G. fuscipes ). We used previously described ACPs in Drosophila melanogaster and Anopheles gambiae as reference sequences. We identified 36, 27, 31, 29 and 33 diverse ACP orthologous genes in G. austeni, G. brevipalpis, G. fuscipes, G. pallidipes and G. morsitans genomes respectively, which we classified into 21 functional classes. Our findings provide genetic evidence of MAG proteins in five recently sequenced Glossina genomes. It highlights new avenues for molecular studies that evaluate potential field control strategies of these important vectors of human and animal disease. Competing Interests: Competing interests: No competing interests were disclosed. (Copyright: © 2017 Abry MF et al.)
Databáze:	MEDLINE
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