Characterization of cellulase from Aspergillus tubingensis NKBP-55 for generation of fermentable sugars from agricultural residues.

Autor: Prajapati BP; Department of Microbiology, Dr. Harisingh Gour Vishwavidyalaya (A Central University), Sagar, MP 470003, India., Kumar Suryawanshi R; Department of Microbiology, Dr. Harisingh Gour Vishwavidyalaya (A Central University), Sagar, MP 470003, India., Agrawal S; Department of Microbiology, Dr. Harisingh Gour Vishwavidyalaya (A Central University), Sagar, MP 470003, India., Ghosh M; Department of Physics, Dr. Harisingh Gour Vishwavidyalaya (A Central University), Sagar, MP 470003, India., Kango N; Department of Microbiology, Dr. Harisingh Gour Vishwavidyalaya (A Central University), Sagar, MP 470003, India. Electronic address: nkango@gmail.com.
Jazyk: angličtina
Zdroj: Bioresource technology [Bioresour Technol] 2018 Feb; Vol. 250, pp. 733-740. Date of Electronic Publication: 2017 Dec 02.
DOI: 10.1016/j.biortech.2017.11.099
Abstrakt: The aim of this work was to characterize cellulase from Aspergillus tubingensis NKBP-55 for generation of fermentable sugars from agricultural residues. The strain produced high titres of cellulase (750 U/gds) on copra meal in solid state fermentation (SSF). The enzyme preparation also showed hemicellulolytic activities (U/gds) viz. endo-mannanase (1023), endo-xylanase (167), β-glucosidase (72) and α-galactosidase (54). Zymography revealed presence of six cellulases, six mannanases and one β-glucosidase. It effectively degraded sugarcane bagasse (SCB) and rice straw (RS) releasing xylose, glucose and cellobiose. One cellulase (Cat 1, Mr ∼65 kDa) was purified and characterized. It retained more than 50% activity at 70 °C after 150 mins and its activity was enhanced in the presence of Mn 2+ ions (130%) and β-mercaptoethanol (140%). FTIR and 13 C CP/MAS NMR analysis of the enzyme treated SCB and RS revealed degradation of cellulose and hemicellulose, while 1 H and 13 C liquid state NMR experiments confirmed release of glucose.
(Copyright © 2017 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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