Anti-inflammatory, antinociceptive and antioxidant properties of Schinopsis brasiliensis bark.

Autor: Santos CCS; Universidade Federal de Sergipe, Departamento de Engenharia Agronômica, Campus Universitário, São Cristóvão, Brasil., Guilhon CC; Universidade Federal do Rio de Janeiro, Instituto de Ciências Biomédicas, Rio de Janeiro, Brasil., Moreno DSA; Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Prof. Paulo de Góes, Rio de Janeiro, Brasil., Alviano CS; Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Prof. Paulo de Góes, Rio de Janeiro, Brasil., Estevam CDS; Universidade Federal de Sergipe, Departamento de Engenharia Agronômica, Campus Universitário, São Cristóvão, Brasil., Blank AF; Universidade Federal de Sergipe, Departamento de Engenharia Agronômica, Campus Universitário, São Cristóvão, Brasil., Fernandes PD; Universidade Federal do Rio de Janeiro, Instituto de Ciências Biomédicas, Rio de Janeiro, Brasil. Electronic address: patricia.dias.icbufrj@gmail.com.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2018 Mar 01; Vol. 213, pp. 176-182. Date of Electronic Publication: 2017 Nov 15.
DOI: 10.1016/j.jep.2017.11.012
Abstrakt: Ethnopharmacological Relevance: Schinopsis brasiliensis is a native plant from Brazil, popularly used in folk medicine to relieve pain and treat inflammation. This study evaluated the antinociceptive and anti-inflammatory activities and antioxidant properties of the hydroethanol extract (HEE) and ethyl acetate fraction (EAF) obtained from S. brasiliensis bark.
Materials and Methods: The HEE and EAF of S. brasiliensis bark (10, 30 and 100mg/kg, p.o.) were evaluated using models of analgaesia (formalin-induced licking and hot-plate models) or inflammation (licking response by formalin-induced and carrageenan-induced cell migration into the subcutaneous air pouch). The antioxidant activities of HEE and EAF (50, 100 and 200µg/ml) were evaluated using the lipoperoxidation method induced in egg yolk by 2'-azobis(2-amidinopropane) dihydrochloride (AAPH) and FeSO 4 .
Results: HEE and EAF presented a central antinociceptive effect (at 100mg/kg dose), increasing the baseline and area under the curve in the hot plate model. EAF (100mg/kg) significantly reduced (p< 0.005) the pain response in the first (45%) and second (35%) phases of the formalin-induced licking model, while HEE (100mg/kg) reduced (38%) only the pain response in the second phase. Regarding anti-inflammatory activity, EAF (100mg/kg) also inhibited the inflammatory process induced by subcutaneous carrageenan injection in the SAP model, reducing the amount of the cytokine TNF-α produced.
Conclusion: HEE and EAF from S. brasiliensis bark show pharmacological interest because they were able to inhibit the peripheral and central transmission of pain. Our data also suggest that the anti-inflammatory activity caused by EAF exposure occurs through the inhibition of the pro-inflammatory cytokine TNF-α, also reducing the spreading of the inflammatory processes by neutralizing reactive oxygen species, which are by-products in the biosynthesis of pain mediators.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: