Initial Steps of Amyloidogenic Peptide Assembly Revealed by Cold-Ion Spectroscopy.

Autor: Ujma J; Michael Barber Centre for Collaborative Mass Spectrometry, Manchester Institute for Biotechnology, University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK., Kopysov V; Laboratoire de Chimie Physique Moléculaire, École Polytechnique Fédérale de Lausanne, 1015, Lausanne, Switzerland., Nagornova NS; Laboratoire de Chimie Physique Moléculaire, École Polytechnique Fédérale de Lausanne, 1015, Lausanne, Switzerland., Migas LG; Michael Barber Centre for Collaborative Mass Spectrometry, Manchester Institute for Biotechnology, University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK., Lizio MG; Michael Barber Centre for Collaborative Mass Spectrometry, Manchester Institute for Biotechnology, University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK., Blanch EW; School of Science, RMIT University, 124a La Trobe Street, Melbourne, VIC, 3001, Australia., MacPhee C; The School of Physics and Astronomy, James Maxwell Clark Building, The University of Edinburgh, Edinburgh, EH9 3FD, UK., Boyarkin OV; Laboratoire de Chimie Physique Moléculaire, École Polytechnique Fédérale de Lausanne, 1015, Lausanne, Switzerland., Barran PE; Michael Barber Centre for Collaborative Mass Spectrometry, Manchester Institute for Biotechnology, University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK.
Jazyk: angličtina
Zdroj: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2018 Jan 02; Vol. 57 (1), pp. 213-217. Date of Electronic Publication: 2017 Dec 07.
DOI: 10.1002/anie.201710188
Abstrakt: The early stages of fibril formation are difficult to capture in solution. We use cold-ion spectroscopy to examine an 11-residue peptide derived from the protein transthyretin and clusters of this fibre-forming peptide containing up to five units in the gas phase. For each oligomer, the UV spectra exhibit distinct changes in the electronic environment of aromatic residues in this peptide compared to that of the monomer and in the bulk solution. The UV spectra of the tetra- and pentamer are superimposable but differ significantly from the spectra of the monomer and trimer. Such a spectral evolution suggests that a common structural motif is formed as early as the tetramer. The presence of this stable motif is further supported by the low conformational heterogeneity of the tetra- and pentamer, revealed from their IR spectra. From comparison of the IR-spectra in the gas and condensed phases, we propose putative assignments for the dominant motif in the oligomers.
(© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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