S -nitroso- and nitro- proteomes in the olive ( Olea europaea L.) pollen. Predictive versus experimental data by nano-LC-MS.

Autor:	Carmona R; Plant Reproductive Biology Laboratory, Department of Biochemistry, Cellular and Molecular Biology of Plants, Estación Experimental del Zaidín (CSIC), Profesor Albareda 1, 18008 Granada, Spain., Jimenez-Quesada MJ; Plant Reproductive Biology Laboratory, Department of Biochemistry, Cellular and Molecular Biology of Plants, Estación Experimental del Zaidín (CSIC), Profesor Albareda 1, 18008 Granada, Spain., Lima-Cabello E; Plant Reproductive Biology Laboratory, Department of Biochemistry, Cellular and Molecular Biology of Plants, Estación Experimental del Zaidín (CSIC), Profesor Albareda 1, 18008 Granada, Spain., Traverso JÁ; Plant Reproductive Biology Laboratory, Department of Biochemistry, Cellular and Molecular Biology of Plants, Estación Experimental del Zaidín (CSIC), Profesor Albareda 1, 18008 Granada, Spain., Castro AJ; Plant Reproductive Biology Laboratory, Department of Biochemistry, Cellular and Molecular Biology of Plants, Estación Experimental del Zaidín (CSIC), Profesor Albareda 1, 18008 Granada, Spain., Claros MG; Departamento de Biología Molecular y Bioquímica, Universidad de Málaga, Málaga, Spain., de Dios Alché J; Plant Reproductive Biology Laboratory, Department of Biochemistry, Cellular and Molecular Biology of Plants, Estación Experimental del Zaidín (CSIC), Profesor Albareda 1, 18008 Granada, Spain.
Jazyk:	angličtina
Zdroj:	Data in brief [Data Brief] 2017 Oct 06; Vol. 15, pp. 474-477. Date of Electronic Publication: 2017 Oct 06 (Print Publication: 2017).
DOI:	10.1016/j.dib.2017.09.058
Abstrakt:	The data presented here are related to the research article entitled "Generation of nitric oxide by olive ( Olea europaea L.) pollen during in vitro germination and assessment of the S -nitroso- and nitro-proteomes by computational predictive methods" doi:10.1016/j.niox.2017.06.005 (Jimenez-Quesada et al., 2017) [1]. Predicted cysteine S -nitrosylation and Tyr-nitration sites in proteins derived from a de novo assembled and annotated pollen transcriptome from olive tree ( Olea europaea L.) were obtained after using well-established predictive tools in silico . Predictions were performed using both default and highly restrictive thresholds. Numerous gene products identified with these characteristics are listed here. An experimental validation of the data, consisting in nano-LC-MS (Liquid Chromatography-Mass Spectrometry) determination of olive pollen proteins after immunoprecipitation with antibodies to anti- S -nitrosoCys and anti-3-NT (NitroTyrosine) allowed identification of numerous proteins subjected to these two post-translational modifications, which are listed here together with information regarding their cross-presence among the predictions.
Databáze:	MEDLINE
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