Multiplexed Detection of MicroRNA Biomarkers Using SERS-Based Inverse Molecular Sentinel (iMS) Nanoprobes.

Autor:	Wang HN; Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, United States.; Fitzpatrick Institute for Photonics, Duke University, Durham, North Carolina 27708, United States., Crawford BM; Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, United States.; Fitzpatrick Institute for Photonics, Duke University, Durham, North Carolina 27708, United States., Fales AM; Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, United States.; Fitzpatrick Institute for Photonics, Duke University, Durham, North Carolina 27708, United States., Bowie ML; Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710, United States., Seewaldt VL; Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, United States., Vo-Dinh T; Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, United States.; Fitzpatrick Institute for Photonics, Duke University, Durham, North Carolina 27708, United States.; Department of Chemistry, Duke University, Durham, North Carolina 27708, United States.
Jazyk:	angličtina
Zdroj:	The journal of physical chemistry. C, Nanomaterials and interfaces [J Phys Chem C Nanomater Interfaces] 2016 Sep 22; Vol. 120 (37), pp. 21047-21050. Date of Electronic Publication: 2016 Jul 01.
DOI:	10.1021/acs.jpcc.6b03299
Abstrakt:	MicroRNAs (miRNAs) have demonstrated great promise as a novel class of biomarkers for early detection of various cancers, including breast cancer. However, due to technical difficulties in detecting these small molecules, miRNAs have not been adopted into routine clinical practice for early diagnostics. Thus, it is important to develop alternative detection strategies that could offer more advantages over conventional methods. Here, we demonstrate the application of a "turn-on" SERS sensing technology, referred to as "inverse Molecular Sentinel (iMS)" nanoprobes, as a homogeneous assay for multiplexed detection of miRNAs. This SERS nanoprobe involves the use of plasmonic-active nanostars as the sensing platform. The "OFF-to-ON" signal switch is based on a nonenzymatic strand-displacement process and the conformational change of stem-loop (hairpin) oligonucleotide probes upon target binding. This technique was previously used to detect a synthetic DNA sequence of interest. In this study, we modified the design of the nanoprobe to be used for the detection of short (22-nt) miRNA sequences. The demonstration of using iMS nanoprobes to detect miRNAs in real biological samples was performed with total small RNA extracted from breast cancer cell lines. The multiplex capability of the iMS technique was demonstrated using a mixture of the two differently labeled nanoprobes to detect miR-21 and miR-34a miRNA biomarkers for breast cancer. The results of this study demonstrate the feasibility of applying the iMS technique for multiplexed detection of short miRNAs molecules. Competing Interests: Notes The authors declare no competing financial interest.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu