A New Cell Separation Method Based on Antibody-Immobilized Nanoneedle Arrays for the Detection of Intracellular Markers.

Autor: Kawamura R; Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) Central 5 , 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan., Miyazaki M; Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology , 2-24-26 Naka-cho, Koganei, Tokyo 184-8588, Japan., Shimizu K; Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology , 2-24-26 Naka-cho, Koganei, Tokyo 184-8588, Japan., Matsumoto Y; Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology , 2-24-26 Naka-cho, Koganei, Tokyo 184-8588, Japan., Silberberg YR; Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) Central 5 , 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan., Sathuluri RR; Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) Central 5 , 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan., Iijima M; Department of Biomolecular Science and Reaction, The Institute of Scientific and Industrial Research (ISIR-Sanken), Osaka University , 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan., Kuroda S; Department of Biomolecular Science and Reaction, The Institute of Scientific and Industrial Research (ISIR-Sanken), Osaka University , 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan., Iwata F; Department of Mechanical Engineering, Shizuoka University , 3-5-1 Johoku, Hamamatsu 432-8561, Japan., Kobayashi T; Research Center for Ubiquitous MEMS and Micro Engineering, AIST , 1-2-1, Namiki, Tsukuba, Ibaraki 305-8564, Japan., Nakamura C; Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) Central 5 , 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.; Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology , 2-24-26 Naka-cho, Koganei, Tokyo 184-8588, Japan.
Jazyk: angličtina
Zdroj: Nano letters [Nano Lett] 2017 Nov 08; Vol. 17 (11), pp. 7117-7124. Date of Electronic Publication: 2017 Oct 26.
DOI: 10.1021/acs.nanolett.7b03918
Abstrakt: Focusing on intracellular targets, we propose a new cell separation technique based on a nanoneedle array (NNA) device, which allows simultaneous insertion of multiple needles into multiple cells. The device is designed to target and lift ("fish") individual cells from a mixed population of cells on a substrate using an antibody-functionalized NNA. The mechanics underlying this approach were validated by force analysis using an atomic force microscope. Accurate high-throughput separation was achieved using one-to-one contacts between the nanoneedles and the cells by preparing a single-cell array in which the positions of the cells were aligned with 10,000 nanoneedles in the NNA. Cell-type-specific separation was realized by controlling the adhesion force so that the cells could be detached in cell-type-independent manner. Separation of nestin-expressing neural stem cells (NSCs) derived from human induced pluripotent stem cells (hiPSCs) was demonstrated using the proposed technology, and successful differentiation to neuronal cells was confirmed.
Databáze: MEDLINE
Externí odkaz: