The sensitivity of Na + , K + ATPase as an indicator of blood diseases.

Autor:	Omar AK; Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.; Experimental Biochemistry unit, King Fahad Medical Research center (KFMRC), Jeddah, Kingdom of Saudi Arabia.; Bioactive Natural Products Research Group, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia., Ahmed KA; Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia., Helmi NM; Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.; Experimental Biochemistry unit, King Fahad Medical Research center (KFMRC), Jeddah, Kingdom of Saudi Arabia., Abdullah KT; Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.; Experimental Biochemistry unit, King Fahad Medical Research center (KFMRC), Jeddah, Kingdom of Saudi Arabia.; Production of bio-products for industrial applications Research group, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia., Qarii MH; Hematology Department, Faculty of Medical Science, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia., Hasan HE; Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia., Ashwag A; Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia., Nabil AM; Consultant cardiologist, King Abdulaziz University Hospital, Jeddah, Kingdom of Saudi Arabia., Abdu AM; Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia., Salama MS; Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.; Experimental Biochemistry unit, King Fahad Medical Research center (KFMRC), Jeddah, Kingdom of Saudi Arabia.; Bioactive Natural Products Research Group, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.; Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
Jazyk:	angličtina
Zdroj:	African health sciences [Afr Health Sci] 2017 Mar; Vol. 17 (1), pp. 262-269.
DOI:	10.4314/ahs.v17i1.32
Abstrakt:	Background: Blood-related hereditary diseases are widespread in Eastern and SouthWestern regions of Saudi Arabia until recently. In this study, we used Na + , K + ATPase as an enzymatic indicator for the diagnosis of the diseases. Materials and Methods: Individuals with different blood diseases (iron deficiency (n=13), anemia (n=14), thalassemia (n=16) and sickle cell anemia (n=12) were studied for Na + , K + -ATPase activity in the plasma membrane of red blood cell and compared with those of the healthy ones (n=20) of the same age and gender living in Jeddah, Saudi Arabia. Results: There was a significant elevation in the specific activity of Na + , K + ATPase in individuals with anemia compared with those of control (0.0094 + 0.001 nmol / mg protein/min versus 0.0061 ± 0.001). On the other hand, there was a significant reduction in enzyme activity in thalassemia (0.0028 ± 0.002 nmol / mg protein/min) and sickle cell anemia cases (0.0042 ±0.001 nmol / mg protein/min) compared to the control group. The cut off value for Na + , K + ATPase activity is 0.005 µmol Pi/min-showing 94% sensitivity and 93% specificity for the differentiation of blood abnormality. Conclusion: It can be recommended that the activity of Na + , K + -ATPase can be used for the diagnosis of individuals with blood diseases/disorders.
Databáze:	MEDLINE
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