Role of the route of leukotrienes in an experimental model of oral mucositis induced by 5-fluorouracil 1.

Autor:	Silva VCD; Fellow PhD degree, Postgraduate Program in Medical and Surgical Sciences, Department of Surgery, School of Medicine, Universidade Federal do Ceará (UFCE), Fortaleza-CE, Brazil. Conception and design of the study, acquisition and interpretation of data, statistics analysis, manuscript preparation, final approval., Leitão RFC; PhD, Postgraduate Program in Morphofunctional Sciences, Department of Morphology, School of Medicine, UFCE, Fortaleza-CE, Brazil. Acquisition and interpretation of data, histopathological examinations, manuscript preparation., Brito GAC; PhD, Postgraduate Program in Morphofunctional Sciences, Department of Morphology, School of Medicine, UFCE, Fortaleza-CE, Brazil. Acquisition and interpretation of data, histopathological examinations, manuscript preparation., Martins CDS; PhD, Postgraduate Program in Morphofunctional Sciences, Department of Morphology, School of Medicine, UFCE, Fortaleza-CE, Brazil. Acquisition and interpretation of data, histopathological examinations, manuscript preparation., Freire GE; Fellow Master degree, Postgraduate Program in Morphofunctional Sciences, Department of Morphology, School of Medicine, UFCE, Fortaleza-CE, Brazil. Acquisition and interpretation of data, manuscript preparation., Aragão KS; PhD, Laboratory of Inflammation and Cancer Pharmacology (LAFICA), UFCE, Fortaleza-CE, Brazil. Conception and design of the study, acquisition and interpretation of data, manuscript preparation., Wanderley CWS; Fellow PhD degree, LAFICA, UFCE, Fortaleza-CE, Brazil. Acquisition and interpretation of data, manuscript preparation., Freitas MR; PhD, Postgraduate Program in Medical and Surgical Sciences, Department of Surgery, Faculty of Medicine, UFCE, Fortaleza-CE, Brazil. Conception and design of the study, interpretation of data, manuscript preparation, final approval.
Jazyk:	angličtina
Zdroj:	Acta cirurgica brasileira [Acta Cir Bras] 2017 Sep; Vol. 32 (9), pp. 712-725.
DOI:	10.1590/s0102-865020170090000004
Abstrakt:	Purpose: To investigate the participation of cysteinyl leukotrienes in the pathophysiology of oral mucositis. Methods: Oral mucositis was induced in hamsters using 5-fluorouracil (5-FU; 60 and 40 mg/kg; i.p., on days 1 and 2, respectively, and with excoriations in jugal mucosa on day 4). Montelukast (10, 20, or 40 mg/kg/d; gavage), MK886 (3 mg/kg/d, i.p.), or saline or celecoxib (7.5 mg/kg/d; i.p.) was administered 1 h prior to 5-FU and daily, until the fourth (MK886) or tenth day, when the animals were euthanized and their jugal mucosa was collected for macroscopic, histopathological, and immunohistochemical evaluation. Results: Neither montelukast nor MK-886 prevented the oral mucositis induced by 5-FU, as observed by histopathological evaluation. In addition, we did not find significant differences in the expression of inducible nitric oxide synthase-2, cyclooxygenase-2, or interleukin (IL)-1β between the experimental and control groups. However, we did observe a significant decrease in tumor necrosis factor (TNF)-α expression for all doses of montelukast; we also observed a significant decrease in IL-10 with 40 mg/kg/d and MK 886. Conclusions: Cysteinyl leukotrienes do not play an important role in experimental oral mucositis induced by 5-FU. There is a modulating action specifically on TNF-α.
Databáze:	MEDLINE
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