Clonality, virulence and antimicrobial resistance of enteroaggregative Escherichia coli from Mirzapur, Bangladesh.

Autor:	Chattaway MA; Gastrointestinal Bacteria Reference Unit, Public Health England, London, UK., Day M; Antimicrobial Resistance and Healthcare Associated Infections, Public Health England, London, UK., Mtwale J; Gastrointestinal Bacteria Reference Unit, Public Health England, London, UK.; Centre for Clinical Microbiology, Division of Infection and Immunity, University of College London, London, UK., White E; Gastrointestinal Bacteria Reference Unit, Public Health England, London, UK.; Department of Medical Microbiology, Royal Free London NHS Foundation Trust, London, UK., Rogers J; Gastrointestinal Bacteria Reference Unit, Public Health England, London, UK., Day M; Gastrointestinal Bacteria Reference Unit, Public Health England, London, UK., Powell D; Gastrointestinal Bacteria Reference Unit, Public Health England, London, UK., Ahmad M; Department of Microbiology and Infection Control, Technical University of Denmark, Denmark., Harris R; Statistics, Modelling and Economics Department, Public Health England, London, UK., Talukder KA; Centre for Food and Water Borne Diseases, International Centre for Diarrhoeal Disease Research, Bangladesh., Wain J; Norwich Medical School, University of East Anglia, Norwich, UK., Jenkins C; Gastrointestinal Bacteria Reference Unit, Public Health England, London, UK., Cravioto A; Centre for Food and Water Borne Diseases, International Centre for Diarrhoeal Disease Research, Bangladesh.
Jazyk:	angličtina
Zdroj:	Journal of medical microbiology [J Med Microbiol] 2017 Oct; Vol. 66 (10), pp. 1429-1435. Date of Electronic Publication: 2017 Sep 25.
DOI:	10.1099/jmm.0.000594
Abstrakt:	Purpose: This study investigates the virulence and antimicrobial resistance in association with common clonal complexes (CCs) of enteroaggregative Escherichia coli (EAEC) isolated from Bangladesh. The aim was to determine whether specific CCs were more likely to be associated with putative virulence genes and/or antimicrobial resistance. Methodology: The presence of 15 virulence genes (by PCR) and susceptibility to 18 antibiotics were determined for 151 EAEC isolated from cases and controls during an intestinal infectious disease study carried out between 2007-2011 in the rural setting of Mirzapur, Bangladesh (Kotloff KL, Blackwelder WC, Nasrin D, Nataro JP, Farag TH et al.Clin Infect Dis 2012;55:S232-S245). These data were then analysed in the context of previously determined serotypes and clonal complexes defined by multi-locus sequence typing. Results: Overall there was no association between the presence of virulence or antimicrobial resistance genes in isolates of EAEC from cases versus controls. However, when stratified by clonal complex (CC) one CC associated with cases harboured more virulence factors (CC40) and one CC harboured more resistance genes (CC38) than the average. There was no direct link between the virulence gene content and antibiotic resistance. Strains within a single CC had variable virulence and resistance gene content indicating independent and multiple gene acquisitions over time. Conclusion: In Bangladesh, there are multiple clonal complexes of EAEC harbouring a variety of virulence and resistance genes. The emergence of two of the most successful clones appeared to be linked to either increased virulence (CC40) or antimicrobial resistance (CC38), but increased resistance and virulence were not found in the same clonal complexes.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu