Ultrasensitive and rapid immuno-detection of human IgE anti-therapeutic horse sera using an electrochemical immunosensor.

Autor: Prado IC; FIOCRUZ, Center for Technological Development in Health (CDTS)/National Institute of Science and Technology on Innovation in Neglected Population Diseases (INCT-IDPN) FIOCRUZ, Rio de Janeiro, RJ, Brazil; Federal Fluminense University, Chemistry Institute, Analytical Chemistry Department, Niterói, Rio de Janeiro, Brazil., Souza ALA; FIOCRUZ, Oswaldo Cruz Institute, Laboratory of Experimental and Computational Biochemistry of Pharmaceuticals, Rio de Janeiro, RJ, Brazil., Provance DW Jr; FIOCRUZ, Center for Technological Development in Health (CDTS)/National Institute of Science and Technology on Innovation in Neglected Population Diseases (INCT-IDPN) FIOCRUZ, Rio de Janeiro, RJ, Brazil., Cassella RJ; Federal Fluminense University, Chemistry Institute, Analytical Chemistry Department, Niterói, Rio de Janeiro, Brazil., De-Simone SG; FIOCRUZ, Center for Technological Development in Health (CDTS)/National Institute of Science and Technology on Innovation in Neglected Population Diseases (INCT-IDPN) FIOCRUZ, Rio de Janeiro, RJ, Brazil; FIOCRUZ, Oswaldo Cruz Institute, Laboratory of Experimental and Computational Biochemistry of Pharmaceuticals, Rio de Janeiro, RJ, Brazil; Federal Fluminense University, Biology Institute, Cellular and Molecular Department, Niterói, Rio de Janeiro, Brazil. Electronic address: dsimone@ioc.fiocruz.br.
Jazyk: angličtina
Zdroj: Analytical biochemistry [Anal Biochem] 2017 Dec 01; Vol. 538, pp. 13-19. Date of Electronic Publication: 2017 Sep 14.
DOI: 10.1016/j.ab.2017.09.008
Abstrakt: Antivenom allergy disease mediated by patient IgE is an important public health care concern. To improve detection of hypersensitive individuals prior to passive antibody therapy, an amperometric immunosensor was developed to detect reactive human IgE. Whole horse IgG3 (hoIgG3) was immobilized onto the surface of carbon or gold screen-printed electrodes through a cross-linking solution of glutaraldehyde on a chitosan film. Sera from persons with a known allergic response to hoIgG3 or non-allergic individuals was applied to the sensor. Bound human IgE (humIgE) was detected by an anti-humIgE antibody through a quantitative amperometric determination by tracking via the electrochemical reduction of the quinone generated from the hydroquinone with the application of a potential of 25 mV. The optimal immunosensor configuration detected reactive humIgE at a dilution of 1:1800 of the human sera that represent a detection limit of 0.5 pg/mL. Stability testing demonstrated that through 20 cycles of a scan, the specificity and performance remained robust. The new immunosensor successfully detected humIgE antibodies reactive against hoIgG3, which could allow the diagnosis of potential allergenic patients needing therapeutic antivenom preparations from a horse.
(Copyright © 2017. Published by Elsevier Inc.)
Databáze: MEDLINE
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