Mucosa-associated microbiota dysbiosis in colitis associated cancer.

Autor: Richard ML; a Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay , Jouy en Josas , France.; b Inflammation-Immunopathology-Biotherapy Département Hospitalo-Universitaire (DHU i2B) , Paris , France., Liguori G; c Department of Medical and Surgical Sciences , University of Bologna , Bologna , Italy., Lamas B; b Inflammation-Immunopathology-Biotherapy Département Hospitalo-Universitaire (DHU i2B) , Paris , France.; d Equipe Avenir Gut Microbiota and Immunity, ERL INSERM U 1157/UMR7203, Faculté de Médecine Saint-Antoine, Université Pierre et Marie Curie (UPMC) , Paris , France., Brandi G; e Department of Experimental , Diagnostic and Specialty Medicine, University of Bologna , Bologna , Italy., da Costa G; a Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay , Jouy en Josas , France.; b Inflammation-Immunopathology-Biotherapy Département Hospitalo-Universitaire (DHU i2B) , Paris , France., Hoffmann TW; a Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay , Jouy en Josas , France.; b Inflammation-Immunopathology-Biotherapy Département Hospitalo-Universitaire (DHU i2B) , Paris , France., Pierluigi Di Simone M; c Department of Medical and Surgical Sciences , University of Bologna , Bologna , Italy., Calabrese C; c Department of Medical and Surgical Sciences , University of Bologna , Bologna , Italy., Poggioli G; c Department of Medical and Surgical Sciences , University of Bologna , Bologna , Italy., Langella P; a Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay , Jouy en Josas , France.; b Inflammation-Immunopathology-Biotherapy Département Hospitalo-Universitaire (DHU i2B) , Paris , France., Campieri M; c Department of Medical and Surgical Sciences , University of Bologna , Bologna , Italy., Sokol H; a Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay , Jouy en Josas , France.; b Inflammation-Immunopathology-Biotherapy Département Hospitalo-Universitaire (DHU i2B) , Paris , France.; d Equipe Avenir Gut Microbiota and Immunity, ERL INSERM U 1157/UMR7203, Faculté de Médecine Saint-Antoine, Université Pierre et Marie Curie (UPMC) , Paris , France.; f Service de Gastroentérologie et Nutrition, Hôpital Saint-Antoine, Assistance Publique Hôpitaux de Paris et Université Paris 6 , Paris , France.
Jazyk: angličtina
Zdroj: Gut microbes [Gut Microbes] 2018 Mar 04; Vol. 9 (2), pp. 131-142. Date of Electronic Publication: 2017 Oct 12.
DOI: 10.1080/19490976.2017.1379637
Abstrakt: Gut microbiota dysbiosis has been associated with inflammatory bowel diseases (IBD). In colorectal cancer, the gut microbiota has also been recognized as potentially involved in aggravating or favoring the tumor development. However, very little is known on the structure and role of the microbiota in colitis associated cancer (CAC), an important complication of IBD in human. Here we analyzed the bacterial and fungal composition of the mucosa associated microbiota of patients suffering CAC, sporadic cancer (SC) and of healthy subjects (HS) by barcode sequences analysis on the following cohort: 7 CAC patients, 10 SC patients and 10 HS using 16S (MiSeq) and ITS2 (pyrosequencing) sequencing, for bacteria and fungi respectively. Mucosa-associated bacterial microbiota in CAC was significantly different from the ones in SC or in HS, while the fungal showed no differences. Comparison between mucosa-associated microbiota on the tumor site or in normal mucosa near the tumor showed very similar patterns. The global mucosa-associated bacterial microbiota in cancer patients was characterized by a restriction in biodiversity but no change for the fungal community. Compared to SC, CAC was characterized by an increase of Enterobacteriacae family and Sphingomonas genus and a decrease of Fusobacterium and Ruminococcus genus. Our study confirms the alteration of the mucosa-associated bacterial microbiota in IBD and SC. Although the cohort is limited in number, this is the first evidence of the existence of an altered bacterial microbiota in CAC clearly different from the one in SC patients.
Databáze: MEDLINE
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