Biosafety level-2 laboratory diagnosis of Zaire Ebola virus disease imported from Liberia to Nigeria.

Autor:	Salu OB; Department of Medical Microbiology and Parasitology, College of Medicine, University of Lagos, Lagos, Nigeria.; Central Research Laboratory, College of Medicine, University of Lagos, Lagos, Nigeria., James AB; Department of Biochemistry, College of Medicine, University of Lagos, Lagos, Nigeria.; Central Research Laboratory, College of Medicine, University of Lagos, Lagos, Nigeria., Oke BO; Department of Medical Microbiology and Parasitology, College of Medicine, University of Lagos, Lagos, Nigeria., Orenolu MR; Central Research Laboratory, College of Medicine, University of Lagos, Lagos, Nigeria., Anyanwu RA; Central Research Laboratory, College of Medicine, University of Lagos, Lagos, Nigeria., Abdullah MA; Central Research Laboratory, College of Medicine, University of Lagos, Lagos, Nigeria., Happi C; African Center of Excellence for Genomics of Infectious Diseases, Redeemers University, Ede, Osun State, Nigeria.; Department of Biological Sciences, College of Natural Sciences, Redeemers University, Ede, Osun State, Nigeria., Idris J; Honourable Commissioner for Health, Lagos State Ministry of Health, Alausa, Ikeja, Lagos, Nigeria., Abdus-Salam IA; Epidemiology Unit, Directorate of Disease Control, Lagos State Ministry of Health, Alausa, Ikeja, Lagos, Nigeria., Nasidi AS; Nigeria Center for Disease Control, Federal Ministry of Health, Abuja, Nigeria., Ogunsola FT; Department of Medical Microbiology and Parasitology, College of Medicine, University of Lagos, Lagos, Nigeria.; Central Research Laboratory, College of Medicine, University of Lagos, Lagos, Nigeria., Tomori O; Nigerian Academy of Science, Lagos, Nigeria., Omilabu SA; Department of Medical Microbiology and Parasitology, College of Medicine, University of Lagos, Lagos, Nigeria.; Central Research Laboratory, College of Medicine, University of Lagos, Lagos, Nigeria.
Jazyk:	angličtina
Zdroj:	African journal of laboratory medicine [Afr J Lab Med] 2016 Oct 17; Vol. 5 (1), pp. 468. Date of Electronic Publication: 2016 Oct 17 (Print Publication: 2016).
DOI:	10.4102/ajlm.v5i1.468
Abstrakt:	Introduction: Global travel is an efficient route of transmission for highly infectious pathogens and increases the chances of such pathogens moving from high disease-endemic areas to new regions. We describe the rapid and safe identification of the first imported case of Ebola virus disease in a traveler to Lagos, Nigeria, using conventional reverse transcription polymerase chain reaction (RT-PCR) in a biosafety level (BSL)-2 facility. Case Presentation: On 20 July 2014, a traveler arrived from Liberia at Lagos International Airport and was admitted to a private hospital in Lagos, with clinical suspicion of Ebola virus disease. Methodology and Outcome: Blood and urine specimens were collected, transported to the Virology Unit Laboratory at the College of Medicine, University of Lagos, and processed under stringent biosafety conditions for viral RNA extraction. RT-PCR was set-up to query the Ebola, Lassa and Dengue fever viruses. Amplicons for pan-filoviruses were detected as 300 bp bands on a 1.5% agarose gel image; there were no detectable bands for Lassa and Dengue viral RNA. Nucleotide BLAST and phylogenetic analysis of sequence data of the RNA-dependent RNA polymerase (L) gene confirmed the sequence to be Zaire ebolavirus (EBOV/Hsap/NGA/2014/LIB-NIG 01072014; Genbank: KM251803.1). Conclusion: Our BSL-2 facility in Lagos, Nigeria, was able to safely detect Ebola virus disease using molecular techniques, supporting the reliability of molecular detection of highly infectious viral pathogens under stringent safety guidelines in BSL-2 laboratories. This is a significant lesson for the many under-facilitated laboratories in resource-limited settings, as is predominantly found in sub-Saharan Africa. Competing Interests: The authors declare that they have no financial or personal relationship(s) which may have inappropriately influenced them in writing this article.
Databáze:	MEDLINE
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