A novel culture method reveals unique neural stem/progenitors in mature porcine iris tissues that differentiate into neuronal and rod photoreceptor-like cells.

Autor: Royall LN; Developmental Neurobiology Laboratory, Department of Biological Sciences, Nara Women's University, Nara 630-8506, Japan; Department of Genetics, University of Leicester, Leicester, UK., Lea D; Developmental Neurobiology Laboratory, Department of Biological Sciences, Nara Women's University, Nara 630-8506, Japan; Department of Genetics, University of Leicester, Leicester, UK., Matsushita T; Developmental Neurobiology Laboratory, Department of Biological Sciences, Nara Women's University, Nara 630-8506, Japan., Takeda TA; Department of Biotechnology, Kyoto Institute of Technology, Kyoto 606-8585, Japan., Taketani S; Department of Biotechnology, Kyoto Institute of Technology, Kyoto 606-8585, Japan., Araki M; Developmental Neurobiology Laboratory, Department of Biological Sciences, Nara Women's University, Nara 630-8506, Japan; Unit of Neural Development and Regeneration Research, Department of Biology, Nara Medical University, Kashihara 634-8521, Japan. Electronic address: araki_masa@naramed-u.ac.jp.
Jazyk: angličtina
Zdroj: Brain research [Brain Res] 2017 Nov 15; Vol. 1675, pp. 51-60. Date of Electronic Publication: 2017 Sep 01.
DOI: 10.1016/j.brainres.2017.08.027
Abstrakt: Iris neural stem/progenitor cells from mature porcine eyes were investigated using a new protocol for tissue culture, which consists of dispase treatment and Matrigel embedding. We used a number of culture conditions and found an intense differentiation of neuronal cells from both the iris pigmented epithelial (IPE) cells and the stroma tissue cells. Rod photoreceptor-like cells were also observed but mostly in a later stage of culture. Neuronal differentiation does not require any additives such as fetal bovine serum or FGF2, although FGF2 and IGF2 appeared to promote neural differentiation in the IPE cultures. Furthermore, the stroma-derived cells were able to be maintained in vitro indefinitely. The evolutionary similarity between humans and domestic pigs highlight the potential for this methodology in the modeling of human diseases and characterizing human ocular stem cells.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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