| Autor: |
Jinesh GG; Department of Urology, The University of Texas MD Anderson Cancer Center, Houston, Texas, 77030, USA., Manyam GC; Department of Bioinformatics, The University of Texas MD Anderson Cancer Center, Houston, Texas, 77030, USA., Mmeje CO; Department of Urology, The University of Texas MD Anderson Cancer Center, Houston, Texas, 77030, USA., Baggerly KA; Department of Bioinformatics, The University of Texas MD Anderson Cancer Center, Houston, Texas, 77030, USA., Kamat AM; Department of Urology, The University of Texas MD Anderson Cancer Center, Houston, Texas, 77030, USA. akamat@mdanderson.org. |
| Abstrakt: |
Cancer cells require both migratory and tumorigenic property to establish metastatic tumors outside the primary microenvironment. Identifying the characteristic features of migratory cancer stem cells with tumorigenic property is important to predict patient prognosis and combat metastasis. Here we established one epithelial and two mesenchymal cell lines from ascites of a bladder cancer patient (i.e. cells already migrated outside primary tumor). Analyses of these cell lines demonstrated that the epithelial cells with surface expression of PD-L1, E-cadherin, CD24, and VEGFR2 rapidly formed tumors outside the primary tumor microenvironment in nude mice, exhibited signatures of immune evasion, increased stemness, increased calcium signaling, transformation, and novel E-cadherin-RalBP1 interaction. The mesenchymal cells on the other hand, exhibited constitutive TGF-β signaling and were less tumorigenic. Hence, targeting epithelial cancer stem cells with rapid tumorigenesis signatures in future might help to combat metastasis. |
