Exposure to atrazine alters behaviour and disrupts the dopaminergic system in Drosophila melanogaster.

Autor: Figueira FH; Programa de Pós-Graduação em Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande - FURG, Av. Itália km 8, Campus Carreiros, 96203-900 Rio Grande, RS, Brazil., de Quadros Oliveira N; Universidade Federal do Rio Grande - FURG, Av. Itália km 8, Campus Carreiros, 96203-900 Rio Grande, RS, Brazil., de Aguiar LM; Programa de Pós-Graduação em Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande - FURG, Av. Itália km 8, Campus Carreiros, 96203-900 Rio Grande, RS, Brazil. Electronic address: lais.mattos@furg.br., Escarrone AL; Universidade Federal do Rio Grande - FURG, Av. Itália km 8, Campus Carreiros, 96203-900 Rio Grande, RS, Brazil., Primel EG; Escola de Química e Alimentos, Universidade Federal do Rio Grande - FURG, Av. Itália km 8, Campus Carreiros, 96203-900 Rio Grande, RS, Brazil. Electronic address: dqmednei@furg.br., Barros DM; Programa de Pós-Graduação em Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande - FURG, Av. Itália km 8, Campus Carreiros, 96203-900 Rio Grande, RS, Brazil., da Rosa CE; Programa de Pós-Graduação em Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande - FURG, Av. Itália km 8, Campus Carreiros, 96203-900 Rio Grande, RS, Brazil. Electronic address: carlosrosa@furg.br.
Jazyk: angličtina
Zdroj: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP [Comp Biochem Physiol C Toxicol Pharmacol] 2017 Nov; Vol. 202, pp. 94-102. Date of Electronic Publication: 2017 Aug 26.
DOI: 10.1016/j.cbpc.2017.08.005
Abstrakt: Atrazine is an extensively used herbicide, and has become a common environmental contaminant. Effects on dopaminergic neurotransmission in mammals following exposure to atrazine have been previously demonstrated. Here, the effects of atrazine regarding behavioural and dopaminergic neurotransmission parameters were assessed in the fruit fly D. melanogaster, exposed during embryonic and larval development. Embryos (newly fertilized eggs) were exposed to two atrazine concentrations (10μM and 100μM) in the diet until the adult fly emerged. Negative geotaxis assay, as well as exploratory behaviour, immobility time and number of grooming episodes in an open field system were assessed. Tyrosine hydroxylase (TH) activity and gene expression of the dopaminergic system were also evaluated in newly emerged male and female flies. All analyzed parameters in male flies were not significantly affected by atrazine exposure. However female flies exposed to atrazine at 10μM presented an increase in immobility time and a reduction in exploratory activity in the open field test, which was offset by an increase in the number of grooming episodes. Also, female flies exposed to 100μM of atrazine presented an increase in immobility time. Gene expression of DOPA decarboxylase and dopamine (DA) receptors were also increased only in females. The behavioural effects of atrazine exposure observed in female flies were due to a disturbance in the dopaminergic system.
(Copyright © 2017 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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