Cutaneous leishmaniosis in naturally infected dogs in Paraná, Brazil, and the epidemiological implications of Leishmania (Viannia) braziliensis detection in internal organs and intact skin.

Autor: Marquez ES; Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná (UFPR), Avenida Coronel Francisco H. dos Santos, 210, Centro Politécnico, Jardim das Américas, Curitiba, PR 81531-970, Brazil; Laboratory of Veterinary Clinical Pathology, Northern Paraná State University (UENP), Rodovia BR 369, Km 54, Vila Maria, Bandeirantes, PR 86360-000, Brazil. Electronic address: esmarquez@uenp.edu.br., de Castro EA; Laboratory of Molecular Parasitology, Federal University of Paraná (UFPR), Avenida Coronel Francisco H. dos Santos, 210, Centro Politécnico, Jardim das Américas, Curitiba, PR 81531-970, Brazil. Electronic address: ecastro@ufpr.br., Nabut LB; Laboratory of Zoonoses, Londrina State University (UEL), Rodovia Celso Garcia Cid, Km 380, s/n, Campus Universitário, Londrina, PR 86057-970, Brazil. Electronic address: biazzono@gmail.com., da Costa-Ribeiro MCV; Laboratory of Molecular Parasitology, Federal University of Paraná (UFPR), Avenida Coronel Francisco H. dos Santos, 210, Centro Politécnico, Jardim das Américas, Curitiba, PR 81531-970, Brazil. Electronic address: magdacostaribeiro@gmail.com., Dela Coletta Troiano Araújo L; Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná (UFPR), Avenida Coronel Francisco H. dos Santos, 210, Centro Politécnico, Jardim das Américas, Curitiba, PR 81531-970, Brazil. Electronic address: lud_yt@hotmail.com., Poubel SB; Laboratory of Molecular Parasitology, Federal University of Paraná (UFPR), Avenida Coronel Francisco H. dos Santos, 210, Centro Politécnico, Jardim das Américas, Curitiba, PR 81531-970, Brazil. Electronic address: saloebispo@gmail.com., Gonçalves AL; Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná (UFPR), Avenida Coronel Francisco H. dos Santos, 210, Centro Politécnico, Jardim das Américas, Curitiba, PR 81531-970, Brazil. Electronic address: andrebios@bol.com.br., Cruz MFR; Laboratory of Zoonoses and Public Health, Northern Paraná State University (UENP), Rodovia BR 369, Km 54, Vila Maria, Bandeirantes, PR 86360-000, Brazil. Electronic address: mfordellone@uenp.edu.br., Dos Santos Trad APME; Laboratory of Histopathology, Northern Paraná State University (UENP), Rodovia BR 369, Km 54, Vila Maria, Bandeirantes, PR 86360-000, Brazil. Electronic address: ap_millet@hotmail.com., Dias RAF; Rolândia Municipal Secretary for Health and Epidemiological Surveillance, Rua Santos Dumont, 704, Centro, Rolândia, PR 86600-109, Brazil. Electronic address: radias@sercomtel.com.br., Navarro IT; Laboratory of Zoonoses, Londrina State University (UEL), Rodovia Celso Garcia Cid, Km 380, s/n, Campus Universitário, Londrina, PR 86057-970, Brazil. Electronic address: italmar@uel.br., Thomaz-Soccol V; Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná (UFPR), Avenida Coronel Francisco H. dos Santos, 210, Centro Politécnico, Jardim das Américas, Curitiba, PR 81531-970, Brazil; Laboratory of Molecular Parasitology, Federal University of Paraná (UFPR), Avenida Coronel Francisco H. dos Santos, 210, Centro Politécnico, Jardim das Américas, Curitiba, PR 81531-970, Brazil. Electronic address: vanetesoccol@gmail.com.
Jazyk: angličtina
Zdroj: Veterinary parasitology [Vet Parasitol] 2017 Aug 30; Vol. 243, pp. 219-225. Date of Electronic Publication: 2017 Jul 05.
DOI: 10.1016/j.vetpar.2017.07.003
Abstrakt: Environmental changes have occurred over the years, altering the eco-epidemiological pattern of leishmaniosis in the State of Paraná, Brazil, involving the pillars of the cycle (parasite, vectors, reservoir, and environment) and their interaction. Much has been discussed about the dog's role as a reservoir of the Leishmania (Viannia) braziliensis Vianna, 1911 transmission cycle. However, this question remains unanswered. The purpose of this study was to investigate, using parasitological and molecular methods, different samples in eight naturally infected dogs from an endemic rural locality where only L. (V.) braziliensis is present, and where human cases have been previously notified. Blood and biopsied organ samples from naturally infected dogs were analyzed by culture media, PCR, random amplified polymorphic DNA and sequencing methodologies. Only skin lesions from all dogs yielded positive cultures and when PCR was performed, L. (V.) braziliensis DNA was amplified from intact skin, peripheral blood, bone marrow, spleen, liver and lymph nodes. RAPD was also applied to isolates from the skin lesions, exhibiting the genetic variability of the parasite identified. To confirm which species of Leishmania was amplified in PCR, the sequencing method was performed, verifying 100% similarity with the Viannia subgenus. This study showed that L. (V.) braziliensis can spread to other sites besides the ulcerous lesions, such as intact skin, peripheral blood and internal organs, making it possibility for dogs to serve as active sources of parasite transmission. For definitive proof, xenodiagnostic test on intact skin of infected dogs, should be done.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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