Effect of a single point mutation on equine herpes virus 9 (EHV-9) neuropathogenicity after intranasal inoculation in a hamster model.

Autor:	Saleh AG; Laboratory of Veterinary Pathology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.; Department of Animal Medicine, Faculty of Veterinary Medicine, Damanhour University, El-Beheira, Egypt., Anwar SI; Department of Pathology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, 62511, Egypt., Abas OM; Laboratory of Veterinary Microbiology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.; Department of Animal Medicine, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt., Abd-Ellatieff HA; Laboratory of Veterinary Pathology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.; Department of Pathology and Parasitology, Faculty of Veterinary Medicine, Damanhour University, El-Beheira, Egypt., Nasr M; Department of Animal Medicine, Faculty of Veterinary Medicine, Damanhour University, El-Beheira, Egypt., Saleh I; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt., Fukushi H; Laboratory of Veterinary Microbiology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan., Yanai T; Laboratory of Veterinary Pathology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Jazyk:	angličtina
Zdroj:	The Journal of veterinary medical science [J Vet Med Sci] 2017 Aug 18; Vol. 79 (8), pp. 1426-1436. Date of Electronic Publication: 2017 Jul 15.
DOI:	10.1292/jvms.17-0076
Abstrakt:	This study aimed to investigate the neuropathogenesis of equine herpes virus 9 (EHV-9) by studying the effects of a single point mutation introduced in two different EHV-9 genes. The two EHV-9 mutants, 14R and 19R, were generated carrying a point mutation in two separate EHV-9 genes. These mutants, along with the wild-type EHV-9, were used to infect a hamster model. The EHV-9- and 19R-infected groups showed earlier and more severe clinical signs of infection than the 14R-infected group. The white blood cells (WBCs) count was significantly increased in both EHV-9- and 19R-infected groups compared to the 14R-infected group at the 4th day post infection (DPI). Viremia was also detected earlier in both EHV-9- and 19R-infected groups than 14R-infected group. There were differences in the anterograde transmission pattern of both EHV-9 and 19R compared to 14R inside the brain. Serum TNF-α, IL-6 and IFN-γ levels were significantly increased in both EHV-9- and 19R-infected groups compared to the 14R-infected group. Histopathological and immunohistochemical analyses revealed that the mean group scores for the entire brain were significantly higher in both EHV-9- and 19R- infected groups than 14R-infected group. Collectively, these results confirm that the gene product of Open Reading Frame 19 (ORF19) plays an important role in EHV-9 neuropathogenicity and that the mutation in ORF19 is responsible for the attenuation of EHV-9.
Databáze:	MEDLINE
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