Emission of volatile organic compounds from petunia flowers is facilitated by an ABC transporter.

Autor: Adebesin F; Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA., Widhalm JR; Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA.; Purdue Center for Plant Biology, Purdue University, West Lafayette, IN 47907, USA., Boachon B; Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA., Lefèvre F; Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud 4-5, Box L7-04-14, 1348 Louvain-la-Neuve, Belgium., Pierman B; Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud 4-5, Box L7-04-14, 1348 Louvain-la-Neuve, Belgium., Lynch JH; Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA., Alam I; Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud 4-5, Box L7-04-14, 1348 Louvain-la-Neuve, Belgium., Junqueira B; Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud 4-5, Box L7-04-14, 1348 Louvain-la-Neuve, Belgium., Benke R; Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA., Ray S; School of Chemical Engineering, Purdue University, 480 Stadium Mall Drive, West Lafayette, IN 47907-2100, USA., Porter JA; Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USA., Yanagisawa M; Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907-2054, USA., Wetzstein HY; Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USA., Morgan JA; Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA.; School of Chemical Engineering, Purdue University, 480 Stadium Mall Drive, West Lafayette, IN 47907-2100, USA., Boutry M; Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud 4-5, Box L7-04-14, 1348 Louvain-la-Neuve, Belgium., Schuurink RC; Department of Plant Physiology, University of Amsterdam, Swammerdam Institute for Life Sciences, Science Park 904, 1098 XH Amsterdam, Netherlands., Dudareva N; Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA. dudareva@purdue.edu.; Purdue Center for Plant Biology, Purdue University, West Lafayette, IN 47907, USA.; Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USA.
Jazyk: angličtina
Zdroj: Science (New York, N.Y.) [Science] 2017 Jun 30; Vol. 356 (6345), pp. 1386-1388.
DOI: 10.1126/science.aan0826
Abstrakt: Plants synthesize a diversity of volatile molecules that are important for reproduction and defense, serve as practical products for humans, and influence atmospheric chemistry and climate. Despite progress in deciphering plant volatile biosynthesis, their release from the cell has been poorly understood. The default assumption has been that volatiles passively diffuse out of cells. By characterization of a Petunia hybrida adenosine triphosphate-binding cassette (ABC) transporter, PhABCG1, we demonstrate that passage of volatiles across the plasma membrane relies on active transport. PhABCG1 down-regulation by RNA interference results in decreased emission of volatiles, which accumulate to toxic levels in the plasma membrane. This study provides direct proof of a biologically mediated mechanism of volatile emission.
(Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.)
Databáze: MEDLINE
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