Evaluation of Biological Activity of Mastic Extracts Based on Chemotherapeutic Indices.

Autor:	Suzuki R; Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, Sakado, Japan ryu_suzu@josai.ac.jp sakagami@dent.meikai.ac.jp., Sakagami H; Meikai University School of Dentistry, Sakado, Japan ryu_suzu@josai.ac.jp sakagami@dent.meikai.ac.jp., Amano S; Meikai University School of Dentistry, Sakado, Japan., Fukuchi K; Graduate School, Showa University, Tokyo, Japan., Sunaga K; Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, Sakado, Japan., Kanamoto T; St. Marianna University School of Medicine, Kawasaki, Japan., Terakubo S; St. Marianna University School of Medicine, Kawasaki, Japan., Nakashima H; St. Marianna University School of Medicine, Kawasaki, Japan., Shirataki Y; Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, Sakado, Japan., Tomomura M; Meikai University School of Dentistry, Sakado, Japan., Masuda Y; Meikai University School of Dentistry, Sakado, Japan., Yokose S; Meikai University School of Dentistry, Sakado, Japan., Tomomura A; Meikai University School of Dentistry, Sakado, Japan., Watanabe H; Sunsho Pharmaceutical Co., Ltd., Fuji, Japan., Okawara M; Sunsho Pharmaceutical Co., Ltd., Fuji, Japan., Matahira Y; Sunsho Pharmaceutical Co., Ltd., Fuji, Japan.
Jazyk:	angličtina
Zdroj:	In vivo (Athens, Greece) [In Vivo] 2017 Jul-Aug; Vol. 31 (4), pp. 591-598.
DOI:	10.21873/invivo.11099
Abstrakt:	Background: Most previous mastic investigators have not considered its potent cytotoxicity that may significantly affect the interpretation of obtained data. In the present study, we re-evaluated several biological activities of mastic extracts, based on chemotherapeutic indexes. Materials and Methods: Pulverized mastic gum was extracted with n-hexane and then with ethyl acetate or independently with methanol or n-butanol. Tumor specificity (TS) of the extracts was determined by their cytotoxicity against human malignant and non-malignant cells. Antibacterial activity was determined by their cytotoxicity against bacteria and normal oral cells. Antiviral activity was determined by their protection of viral infection and cytotoxic activity. Cytochrome P-450 (CYP) 3A4 activity was measured by β-hydroxylation of testosterone. Results: Ethyl acetate extract showed slightly higher tumor specificity (TS=2.6) and one order higher antibacterial activity (selectivity index (SI)=0.813) than other extracts (TS=1.4-2.5; SI=0.030-0.063). All extracts showed no anti-human immunodeficiency virus (HIV) activity, but some anti-herpes simplex virus (HSV) activity, which was masked by potent cytotoxicity. They showed strong inhibitory activity against CYP3A4. Conclusion: Ethyl acetate extraction following the removal of cytotoxic and CYP3A4 inhibitory substances by n-hexane can enhance antitumor and antibacterial activity of mastic. (Copyright© 2017, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
Databáze:	MEDLINE
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