| Autor: |
Losano JDA; Department of Animal Reproduction, Faculty of Veterinary Medicine, University of São Paulo, São Paulo, SP, Brazil., Padín JF; Department of Pharmacology, Faculty of Medicine, Autonomous University of Madrid, Madrid, Spain.; Department of Medical Science, Faculty of Medicine Ciudad Real, University of Castilla-La Mancha, Ciudad Real, Spain., Méndez-López I; Department of Pharmacology, Faculty of Medicine, Autonomous University of Madrid, Madrid, Spain., Angrimani DSR; Department of Animal Reproduction, Faculty of Veterinary Medicine, University of São Paulo, São Paulo, SP, Brazil., García AG; Department of Pharmacology, Faculty of Medicine, Autonomous University of Madrid, Madrid, Spain., Barnabe VH; Department of Animal Reproduction, Faculty of Veterinary Medicine, University of São Paulo, São Paulo, SP, Brazil., Nichi M; Department of Animal Reproduction, Faculty of Veterinary Medicine, University of São Paulo, São Paulo, SP, Brazil.; School of Veterinary Medicine and Animal Science, Avenue Orlando Marques de Paiva, No. 87, 05508-270 São Paulo, SP, Brazil. |
| Abstrakt: |
Studies have reported the importance of mitochondria in sperm functionality. However, for some species, the glycolytic pathway appears to be as important as oxidative phosphorylation in ATP synthesis and sperm kinetics. These mechanisms have not been fully elucidated for bovine spermatozoa. Therefore, the aim of this study was to evaluate the role of mitochondria and the glycolytic pathway in ATP synthesis, sperm movement patterns, and oxidative homeostasis of epididymal spermatozoa in bovine specimens. We observed that mitochondrial uncoupling with protonophores significantly reduced ATP levels. However, these levels were reestablished after stimulation of the glycolytic pathway. We verified the same pattern of results for sperm kinetic variables and the production of reactive oxygen species (ROS). Thus, we suggest that, after its appropriate stimulation, the glycolytic pathway is capable of maintaining ATP levels, sperm kinetic patterns, and oxidative balance of bovine epididymal spermatozoa submitted to mitochondrial uncoupling. |
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