IFT trains in different stages of assembly queue at the ciliary base for consecutive release into the cilium.

Autor: Wingfield JL; Department of Cellular Biology, University of Georgia, Athens, United States., Mengoni I; Department of Cellular Biology, University of Georgia, Athens, United States., Bomberger H; Department of Cellular Biology, University of Georgia, Athens, United States.; College of Engineering, University of Georgia, Athens, United States., Jiang YY; Department of Cellular Biology, University of Georgia, Athens, United States., Walsh JD; Department of Genetics, University of Georgia, Athens, United States., Brown JM; Department of Cell and Developmental Biology, University of Massachusetts Medical School, Worcester, United States.; Department of Biology, Salem State University, Salem, United States., Picariello T; Department of Cell and Developmental Biology, University of Massachusetts Medical School, Worcester, United States., Cochran DA; Department of Cell and Developmental Biology, University of Massachusetts Medical School, Worcester, United States., Zhu B; MOE Key Laboratory of Protein Sciences, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China., Pan J; MOE Key Laboratory of Protein Sciences, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China.; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China., Eggenschwiler J; Department of Genetics, University of Georgia, Athens, United States., Gaertig J; Department of Cellular Biology, University of Georgia, Athens, United States., Witman GB; Department of Cell and Developmental Biology, University of Massachusetts Medical School, Worcester, United States., Kner P; College of Engineering, University of Georgia, Athens, United States., Lechtreck K; Department of Cellular Biology, University of Georgia, Athens, United States.
Jazyk: angličtina
Zdroj: ELife [Elife] 2017 May 31; Vol. 6. Date of Electronic Publication: 2017 May 31.
DOI: 10.7554/eLife.26609
Abstrakt: Intraflagellar transport (IFT) trains, multimegadalton assemblies of IFT proteins and motors, traffic proteins in cilia. To study how trains assemble, we employed fluorescence protein-tagged IFT proteins in Chlamydomonas reinhardtii . IFT-A and motor proteins are recruited from the cell body to the basal body pool, assembled into trains, move through the cilium, and disperse back into the cell body. In contrast to this 'open' system, IFT-B proteins from retrograde trains reenter the pool and a portion is reused directly in anterograde trains indicating a 'semi-open' system. Similar IFT systems were also observed in Tetrahymena thermophila and IMCD3 cells. FRAP analysis indicated that IFT proteins and motors of a given train are sequentially recruited to the basal bodies. IFT dynein and tubulin cargoes are loaded briefly before the trains depart. We conclude that the pool contains IFT trains in multiple stages of assembly queuing for successive release into the cilium upon completion.
Databáze: MEDLINE