| Autor: |
Pando JM; a Department of Biology, New Mexico State University, Las Cruces, NM 88003, USA., Pfeltz RF; b BD Diagnostic Systems, Microbiology R&D Department, Sparks, MD 21152, USA., Cuaron JA; a Department of Biology, New Mexico State University, Las Cruces, NM 88003, USA., Nagarajan V; c Department of Biological Sciences, University of Southern Mississippi, Hattiesburg, MS 39406, USA., Mishra MN; d Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA., Torres NJ; d Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA., Elasri MO; c Department of Biological Sciences, University of Southern Mississippi, Hattiesburg, MS 39406, USA., Wilkinson BJ; e Microbiology Group, School of Biological Sciences, Illinois State University, Normal, IL 61790, USA., Gustafson JE; a Department of Biology, New Mexico State University, Las Cruces, NM 88003, USA.; d Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA. |
| Abstrakt: |
Transcriptional profiles of 2 unrelated clinical methicillin-resistant Staphylococcus aureus (MRSA) isolates were analyzed following 10% (v/v) ethanol challenge (15 min), which arrested growth but did not reduce viability. Ethanol-induced stress (EIS) resulted in differential gene expression of 1091 genes, 600 common to both strains, of which 291 were upregulated. With the exception of the downregulation of genes involved with osmotic stress functions, EIS resulted in the upregulation of genes that contribute to stress response networks, notably those altered by oxidative stress, protein quality control in general, and heat shock in particular. In addition, genes involved with transcription, translation, and nucleotide biosynthesis were downregulated. relP, which encodes a small alarmone synthetase (RelP), was highly upregulated in both MRSA strains following ethanol challenge, and relP inactivation experiments indicated that this gene contributed to EIS growth arrest. A number of persistence-associated genes were also upregulated during EIS, including those that encode toxin-antitoxin systems. Overall, transcriptional profiling indicated that the MRSA investigated responded to EIS by entering a state of dormancy and by altering the expression of elements from cross protective stress response systems in an effort to protect preexisting proteins. |
