Molecular characterization and antibiotic resistance of Staphylococcus spp. isolated from cheese processing plants.

Autor:	Rodrigues MX; Department of Agroindustry, Food and Nutrition, Luiz de Queiroz College of Agriculture, University of São Paulo, Piracicaba, São Paulo, 13418-900, Brazil. Electronic address: marjoryxavier@usp.br., Silva NCC; Department of Agroindustry, Food and Nutrition, Luiz de Queiroz College of Agriculture, University of São Paulo, Piracicaba, São Paulo, 13418-900, Brazil; Department of Food Science, University of Campinas, Campinas, São Paulo, 13083-862, Brazil., Trevilin JH; Department of Agroindustry, Food and Nutrition, Luiz de Queiroz College of Agriculture, University of São Paulo, Piracicaba, São Paulo, 13418-900, Brazil., Cruzado MMB; Department of Agroindustry, Food and Nutrition, Luiz de Queiroz College of Agriculture, University of São Paulo, Piracicaba, São Paulo, 13418-900, Brazil., Mui TS; Cell and Molecular Biology Laboratory, Center for Nuclear Energy in Agriculture, University of São Paulo, Piracicaba, São Paulo, 13400-970, Brazil., Duarte FRS; Cell and Molecular Biology Laboratory, Center for Nuclear Energy in Agriculture, University of São Paulo, Piracicaba, São Paulo, 13400-970, Brazil., Castillo CJC; Department of Agroindustry, Food and Nutrition, Luiz de Queiroz College of Agriculture, University of São Paulo, Piracicaba, São Paulo, 13418-900, Brazil., Canniatti-Brazaca SG; Department of Agroindustry, Food and Nutrition, Luiz de Queiroz College of Agriculture, University of São Paulo, Piracicaba, São Paulo, 13418-900, Brazil., Porto E; Department of Agroindustry, Food and Nutrition, Luiz de Queiroz College of Agriculture, University of São Paulo, Piracicaba, São Paulo, 13418-900, Brazil.
Jazyk:	angličtina
Zdroj:	Journal of dairy science [J Dairy Sci] 2017 Jul; Vol. 100 (7), pp. 5167-5175. Date of Electronic Publication: 2017 Apr 27.
DOI:	10.3168/jds.2016-12477
Abstrakt:	The aim of this research paper was to characterize coagulase-positive and coagulase-negative staphylococci from raw milk, Minas cheese, and production lines of Minas cheese processing. One hundred isolates from 3 different cheese producers were characterized using molecular approaches, such as PCR, molecular typing, and DNA sequencing. Staphylococcus aureus (88% of the isolates) was the most abundant followed by Staphylococcus epidermidis, Staphylococcus hyicus, and Staphylococcus warneri. Among the 22 enterotoxin genes tested, the most frequent was seh (62% of the isolates), followed by selx and ser. Hemolysin genes were widely distributed across isolates, and Panton-Valentine leukocidin and toxic shock syndrome toxin genes were also identified. Methicillin-resistant S. aureus were staphylococcal cassette chromosome mec III, IVa, IVd, and others nontypeable. In the phenotypic antibiotic resistance, multiresistant isolates were detected and resistance to penicillin was the most observed. Using spa typing, we identified several types and described a new one, t14969, isolated from cheese. These findings suggest that antibiotic resistance and potentially virulent strains from different sources can be found in the Brazilian dairy processing environment. Further research should be conducted with collaboration from regulatory agencies to develop programs of prevention of virulent and resistant strain dissemination in dairy products and the processing environment. (Copyright © 2017 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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