Application of an Acyl-CoA Ligase from Streptomyces aizunensis for Lactam Biosynthesis.

Autor:	Zhang J; UCSF-UCB Joint Graduate Group in Bioengineering, University of California , Berkeley, California 94720, United States.; Joint BioEnergy Institute , Emeryville, California 94608, United States.; Synthetic Biology Engineering Research Center, University of California , Berkeley, California 94720, United States., Barajas JF; Joint BioEnergy Institute , Emeryville, California 94608, United States., Burdu M; Joint BioEnergy Institute , Emeryville, California 94608, United States., Wang G; Joint BioEnergy Institute , Emeryville, California 94608, United States., Baidoo EE; Joint BioEnergy Institute , Emeryville, California 94608, United States., Keasling JD; UCSF-UCB Joint Graduate Group in Bioengineering, University of California , Berkeley, California 94720, United States.; Joint BioEnergy Institute , Emeryville, California 94608, United States.; Synthetic Biology Engineering Research Center, University of California , Berkeley, California 94720, United States.; Department of Chemical & Biomolecular Engineering, University of California , Berkeley, California 94720, United States.; California Institute for Quantitative Biosciences, University of California , Berkeley, California 94720, United States.; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark , Kogle Allé, DK2970 Hørsholm, Denmark.
Jazyk:	angličtina
Zdroj:	ACS synthetic biology [ACS Synth Biol] 2017 May 19; Vol. 6 (5), pp. 884-890. Date of Electronic Publication: 2017 Apr 30.
DOI:	10.1021/acssynbio.6b00372
Abstrakt:	ε-Caprolactam and δ-valerolactam are important commodity chemicals used in the manufacture of nylons, with millions of tons produced annually. Biological production of these highly valued chemicals has been limited due to a lack of enzymes that cyclize ω-amino fatty acid precursors to corresponding lactams under ambient conditions. In this study, we demonstrated production of these chemicals using ORF26, an acyl-CoA ligase involved in the biosynthesis of ECO-02301 in Streptomyces aizunensis. This enzyme has a broad substrate spectrum and can cyclize 4-aminobutyric acid into γ-butyrolactam, 5-aminovaleric acid into δ-valerolactam and 6-aminocaproic acid into ε-caprolactam. Recombinant E. coli expressing ORF26 produced valerolactam and caprolactam when 5-aminovaleric acid and 6-aminocaproic acid were added to the culture medium. Upon coexpressing ORF26 with a metabolic pathway that produced 5-aminovaleric acid from lysine, we were able to demonstrate production of δ-valerolactam from lysine.
Databáze:	MEDLINE
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