| Autor: |
Valencia Rivero KT; a Department of Biomedical Engineering , Universidad de los Andes , Bogotá , Colombia., Jaramillo Escobar J; a Department of Biomedical Engineering , Universidad de los Andes , Bogotá , Colombia., Galvis Forero SD; b ANESTCOL SAS , Bogotá , Colombia., Miranda Saldaña MC; c Department of Noninvasive Vascular Diagnosis , Fundacion Cardio Infantil , Bogotá , Colombia., López Panqueva RDP; a Department of Biomedical Engineering , Universidad de los Andes , Bogotá , Colombia.; d Department of Pathology , Fundacion Santa Fe de Bogota , Bogotá , Colombia., Sandoval Reyes NF; e Department of Congenital Heart Disease and Pediatric Cardiovascular Surgery , Fundacion Cardio Infantil , Bogotá , Colombia., Briceño Triana JC; a Department of Biomedical Engineering , Universidad de los Andes , Bogotá , Colombia. |
| Abstrakt: |
The purpose of this study was to evaluate a suitable animal model for the in vivo evaluation of patency and vascular tissue regeneration in small intestinal submucosa (SIS) vascular grafts for hemodialysis access. First, a 4-mm U-shaped SIS vascular graft was implanted between the internal carotid artery (CA) and the external jugular vein (JV) in five sheep and six swine. The U-shape grafts remained functional for 53 ± 4 days in sheep and 32 ± 2 days in swine. The sheep model presented exaggerated inflammation, so the swine model was selected for the in vivo study. Based on these initial results, a 4-mm C-shape SIS vascular graft with SIS circumferential reinforcement was developed to mechanically improve the vascular graft and manage complications identified during surgery in both sheep and swine. The C-shape vascular graft was implanted in a swine model (n = 3) between the CA and JV. GORE-TEX® vascular grafts were used as controls in the contralateral side of the neck. C-shape grafts remained patent for 47 ± 4 days, whereas the GORE-TEX® grafts were patent for 30 ± 15 days. The C-shape vascular graft was easier to handle during surgery, and its circumferential reinforcement improved in vivo patency, avoiding kinks in the graft after implantation. Histological results showed neovascularization and some regeneration with the alignment of endothelial cells in the vascular wall of the grafts. The model developed may be helpful in other research involving in vivo studies of vascular grafts for hemodialysis access. |
