Hydroalcoholic crude extract of Casearia sylvestris Sw. reduces chronic post-ischemic pain by activation of pro-resolving pathways.

Autor: Piovezan AP; Post-Graduate Programm in Health Science - Southern Univeristy of Santa Catarina (UNISUL), Brazil; Laboratory of Experimental Neuroscience (LANEX)- UNISUL, Brazil; William Harvey Research Institute - Queen Mary University of London/London, UK. Electronic address: anna.piovezan@unisul.br., Batisti AP; Post-Graduate Programm in Health Science - Southern Univeristy of Santa Catarina (UNISUL), Brazil; Laboratory of Experimental Neuroscience (LANEX)- UNISUL, Brazil. Electronic address: anapaulabatisti@hotmail.com., Benevides MLACS; Laboratory of Experimental Neuroscience (LANEX)- UNISUL, Brazil; Undergraduation in Medicine - UNISUL, Brazil. Electronic address: marialb994@hotmail.com., Turnes BL; Laboratory of Neurobiology of Pain and Inflammation - UFSC, Brazil. Electronic address: brunalenferss@hotmail.com., Martins DF; Post-Graduate Programm in Health Science - Southern Univeristy of Santa Catarina (UNISUL), Brazil; Laboratory of Experimental Neuroscience (LANEX)- UNISUL, Brazil. Electronic address: danielmartinsfisio@hotmail.com., Kanis L; Post-Graduate Programm in Health Science - Southern Univeristy of Santa Catarina (UNISUL), Brazil. Electronic address: luizalbertokanis@gmail.com., Duarte ECW; Department of Morphological Science - UFSC, Brazil. Electronic address: ijawinkel@yahoo.com.br., Cavalheiro AJ; Department of Organic Chemistry/Institute of Chemistry - UNESP, Brazil. Electronic address: albjcava@gmail.com., Bueno PCP; Department of Organic Chemistry/Institute of Chemistry - UNESP, Brazil. Electronic address: paulabueno@yahoo.com., Seed MP; Clinical Research Group, School of Health Sport & Bioscience, University of East London, UK. Electronic address: m.p.seed@uel.ac.uk., Norling LV; William Harvey Research Institute - Queen Mary University of London/London, UK. Electronic address: l.v.norling@qmul.ac.uk., Cooper D; William Harvey Research Institute - Queen Mary University of London/London, UK. Electronic address: d.cooper@qmul.ac.uk., Headland S; William Harvey Research Institute - Queen Mary University of London/London, UK. Electronic address: sarah.Headland@ucsf.edu., Souza PRPS; William Harvey Research Institute - Queen Mary University of London/London, UK. Electronic address: p.soaresdesouza@qmul.ac.uk., Perretti M; William Harvey Research Institute - Queen Mary University of London/London, UK. Electronic address: m.perretti@qmul.ac.uk.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2017 May 23; Vol. 204, pp. 179-188. Date of Electronic Publication: 2017 Apr 13.
DOI: 10.1016/j.jep.2017.03.059
Abstrakt: Ethnopharmacological Relevance: Casearia sylvestris Sw. is widely used in popular medicine to treat conditions associated with pain.
Aim of the Study: The present study investigated the influence of hydroalcoholic crude extract of Casearia sylvestris (HCE-CS) and contribution of pro-resolving mediators on mechanical hyperalgesia in a mouse model of chronic post-ischemia pain (CPIP).
Methods and Results: Male Swiss mice were subjected to ischemia of the right hind paw (3h), then reperfusion was allowed. At 10min, 24h or 48h post-ischemia/reperfusion (I/R), different groups of animals were treated with HCE-CS (30mg/Kg, orally [p.o]), selected agonists at the pro-resolving receptor ALX/FPR2 (natural molecules like resolvin D1 and lipoxin A4 or the synthetic compound BML-111; 0.1-1µg/animal) or vehicle (saline, 10mL/Kg, s.c.), in the absence or presence of the antagonist WRW4 (10µg, s.c.). Mechanical hyperalgesia (paw withdrawal to von Frey filament) was asseseed together with histological and immunostainning analyses. In these settings, pro-resolving mediators reduced mechanical hyperalgesia and HCE-CS or BML-111 displayed anti-hyperalgesic effects which was markedly attenuated in animals treated with WRW4. ALX/FPR2 expression was raised in skeletal muscle or neutrophils after treatment with HCE-CS or BML-111.
Conclusion: These results reveal significant antihyperalgesic effect of HCE-CS on CPIP, mediated at least in part, by the pathway of resolution of inflammation centred on the axis modulated by ALX/FPR2.
(Copyright © 2017 Elsevier Ireland Ltd. All rights reserved.)
Databáze: MEDLINE
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