Direct labeling of serum proteins by fluorescent dye for antibody microarray.

Autor: Klimushina MV; National Research Center for Preventive Medicine of the Ministry of Healthcare of the Russian Federation, Moscow, 101990, Russian Federation., Gumanova NG; National Research Center for Preventive Medicine of the Ministry of Healthcare of the Russian Federation, Moscow, 101990, Russian Federation. Electronic address: gumanova@mail.ru., Metelskaya VA; National Research Center for Preventive Medicine of the Ministry of Healthcare of the Russian Federation, Moscow, 101990, Russian Federation.
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2017 May 06; Vol. 486 (3), pp. 824-826. Date of Electronic Publication: 2017 Mar 27.
DOI: 10.1016/j.bbrc.2017.03.136
Abstrakt: Analysis of serum proteome by antibody microarray is used to identify novel biomarkers and to study signaling pathways including protein phosphorylation and protein-protein interactions. Labeling of serum proteins is important for optimal performance of the antibody microarray. Proper choice of fluorescent label and optimal concentration of protein loaded on the microarray ensure good quality of imaging that can be reliably scanned and processed by the software. We have optimized direct serum protein labeling using fluorescent dye Arrayit Green 540 (Arrayit Corporation, USA) for antibody microarray. Optimized procedure produces high quality images that can be readily scanned and used for statistical analysis of protein composition of the serum.
(Copyright © 2017 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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