A rapid procedure for the in situ assay of periplasmic, PQQ-dependent methanol dehydrogenase in intact single bacterial colonies.

Autor: Vemuluri VR; Department of Bioenergetics, Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, Pfaffenwaldring 57, D-70569 Stuttgart, Germany; Microbial Culture Collection, National Centre for Cell Science, Pune, Maharashtra 411021, India., Shaw S; Department of Bioenergetics, Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, Pfaffenwaldring 57, D-70569 Stuttgart, Germany., Autenrieth C; Department of Bioenergetics, Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, Pfaffenwaldring 57, D-70569 Stuttgart, Germany., Ghosh R; Department of Bioenergetics, Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, Pfaffenwaldring 57, D-70569 Stuttgart, Germany. Electronic address: robin.ghosh@bio.uni-stuttgart.de.
Jazyk: angličtina
Zdroj: Journal of microbiological methods [J Microbiol Methods] 2017 Jun; Vol. 137, pp. 46-49. Date of Electronic Publication: 2017 Mar 23.
DOI: 10.1016/j.mimet.2017.03.013
Abstrakt: Mechanistic details of methanol oxidation catalyzed by the periplasmically-located pyrroloquinoline quinone-dependent methanol dehydrogenase of methylotrophs can be elucidated using site-directed mutants. Here, we present an in situ colony assay of methanol dehydrogenase, which allows robotic screening of large populations of intact small colonies, and regrowth of colonies for subsequent analysis.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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