STAT5 signaling in kisspeptin cells regulates the timing of puberty.

Autor: Silveira MA; Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil., Furigo IC; Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil., Zampieri TT; Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil., Bohlen TM; Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil., de Paula DG; Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil., Franci CR; Department of Physiology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil., Donato J Jr; Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil., Frazao R; Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil. Electronic address: rfrazao@usp.br.
Jazyk: angličtina
Zdroj: Molecular and cellular endocrinology [Mol Cell Endocrinol] 2017 Jun 15; Vol. 448, pp. 55-65. Date of Electronic Publication: 2017 Mar 23.
DOI: 10.1016/j.mce.2017.03.024
Abstrakt: Previous studies have shown that kisspeptin neurons are important mediators of prolactin's effects on reproduction. However, the cellular mechanisms recruited by prolactin to affect kisspeptin neurons remain unknown. Using whole-cell patch-clamp recordings of brain slices from kisspeptin reporter mice, we observed that 20% of kisspeptin neurons in the anteroventral periventricular nucleus was indirectly depolarized by prolactin via an unknown population of prolactin responsive neurons. This effect required the phosphatidylinositol 3-kinase signaling pathway. No effects on the activity of arcuate kisspeptin neurons were observed, despite a high percentage (70%) of arcuate neurons expressing prolactin-induced STAT5 phosphorylation. To determine whether STAT5 expression in kisspeptin cells regulates reproduction, mice carrying Stat5a/b inactivation specifically in kisspeptin cells were generated. These mutants exhibited an early onset of estrous cyclicity, indicating that STAT5 transcription factors exert an inhibitory effect on the timing of puberty.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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