| Autor: |
Habeeb SM; Parasitology and Animal Diseases Department, Veterinary Research Division, National Research Centre, Post Box: 12622, El Bohouth Street, Dokki, Cairo, Egypt., Ashry HM; Parasitology and Animal Diseases Department, Veterinary Research Division, National Research Centre, Post Box: 12622, El Bohouth Street, Dokki, Cairo, Egypt., Saad MM; Microbial Chemistry Department, National Research Centre, P.O. Box 12622, El-Bohouth Street, Dokki, Cairo, Egypt. |
| Abstrakt: |
The aim of this study was to evaluate the effect of chitinase and protease enzymes produced by environmentally safe soil Fungi; Aspergillus sp. NRC 4/5H; Mucor sp. NRC 5; Trichoderma sp. NRC 4/56; Aspergillus sojae ; Mucor ranosisinus ; Aspergillus oryzae on embryo development of the camel tick Hyalomma dromedarii eggs. The experiment was carried out on the 7 day aged eggs. Concentrations of the chitinase and protease crude enzymes [1:1, 1:2, 1:3, 1:4 and 1:5 (v/v)] were prepared from six stock solutions whose concentrations were 5 units/ml each. The prepared chitinase and protease enzymes produced by soil fungi were tested in vitro study for eradication of ticks. Ovicidal effect of the chitin concentrations of extracellular lytic enzymes (chitinase) produced by Aspergillus sp NRC 4/5H, Mucor sp. NRC 5, Trichoderma sp. NRC 4/56 were tested on H. dromedarii eggs. The results showed that the ovicidal effect increased with increasing the chitin concentration in case of Mucor sp. NRC 5. The maximum inhibitory effect which ranged from 95.3 to 100 % was at concentrations ranging from 1:3 to 1:5 ml/ml, respectively. The results of protease enzymes produced by A. sojae , M. ranosisinus and A. oryzae revealed that, it is highly effective in all concentrations on H . dromedarii eggs. It is indicated that the chitinase and protease enzymes produced by fungal species could be used for biological control of the camel tick infestation to avoid the use of carcinogenic chemical. |
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