| Autor: |
Vieira LFA; Laboratory of Cell Biology, Institute of Biological Sciences and Health, Federal University of Alagoas, CEP 57072-970, Maceió, Alagoas, Brazil., Lins MP; Laboratory of Cell Biology, Institute of Biological Sciences and Health, Federal University of Alagoas, CEP 57072-970, Maceió, Alagoas, Brazil., Viana IMMN; Laboratory of Cell Biology, Institute of Biological Sciences and Health, Federal University of Alagoas, CEP 57072-970, Maceió, Alagoas, Brazil., Dos Santos JE; Laboratory of Cell Biology, Institute of Biological Sciences and Health, Federal University of Alagoas, CEP 57072-970, Maceió, Alagoas, Brazil., Smaniotto S; Laboratory of Cell Biology, Institute of Biological Sciences and Health, Federal University of Alagoas, CEP 57072-970, Maceió, Alagoas, Brazil., Reis MDDS; Laboratory of Cell Biology, Institute of Biological Sciences and Health, Federal University of Alagoas, CEP 57072-970, Maceió, Alagoas, Brazil. danielma.reis@icbs.ufal.br. |
| Abstrakt: |
Nanoparticles have extremely wide applications in the medical and biological fields. They are being used in biosensors, local drug delivery, diagnostics, and medical therapy. However, the potential effects of nanoparticles on target cell and tissue function, apart from cytotoxicity, are not completely understood. Thus, the aim of this study was to investigate the in vitro effects of silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) on human fibroblasts with respect to their interaction with the extracellular matrix and in cell migration. Immunofluorescence analysis revealed that treatment with AgNPs or AuNPs decreased collagen and laminin production at all the concentrations tested (0.1, 1, and 10 μg/mL). Furthermore, cytofluorometric analysis showed that treatment with AgNPs reduced the percentage of cells expressing the collagen receptor very late antigen 2, α 2 β 1 integrin (VLA-2) and the laminin receptor very late antigen 6, α 6 β 1 integrin (VLA-6). In contrast, AuNP treatment increased and decreased the percentages of VLA-2-positive and VLA-6-positive cells, respectively, as compared to the findings for the controls. Analysis of cytoskeletal reorganization showed that treatment with both types of nanoparticles increased the formation of stress fibres and number of cell protrusions and impaired cell polarity. Fibroblasts exposed to different concentrations of AuNPs and AgNPs showed reduced migration through transwell chambers in the functional chemotaxis assay. These results demonstrated that metal nanoparticles may influence fibroblast function by negatively modulating the deposition of extracellular matrix molecules (ECM) and altering the expression of ECM receptors, cytoskeletal reorganization, and cell migration. |
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